Search for progestin receptors (PR) in prolactin-releasing peptidergic neurons: Oxytocin neurons lack PR, but respond to gonadal steroids in monkeys

Cynthia Bethea, Steven Kohama, A. A. Widmann

    Research output: Contribution to journalArticle

    17 Citations (Scopus)

    Abstract

    Progesterone (P) increases PRL secretion in estrogen (E)-primed primates, but not by a direct action on lactotropes. Oxytocin is one of several hypothalamic hormones that stimulate PRL secretion. This study was conducted to determine whether oxytocin neurons directly mediate the action of P on PRL secretion. Hypothalamic sections from steroid-manipulated macaques were double immunolabeled for oxytocin and progestin receptors (PR). In addition, serum levels of oxytocin were measured in steroid-treated macaques, and hypothalamic levels of oxytocin were measured in monkeys under various physiological conditions. E treatment (28 days) of spayed monkeys caused a significant increase in the number of PR-positive neurons in the preoptic area, ventromedial nucleus, arcuate nucleus, and median eminence. Addition of P to the E treatment for the last 14 of 28 days did not change the number of PR-positive neurons in these areas. The number of PR-positive neurons was low and was unchanged by steroid treatment in the supraoptic and rostral paraventricular nuclei. Oxytocin neurons rarely contained PR regardless of anatomical location, steroid treatment, or fixation protocol. Serum oxytocin levels increased with E treatment and increased further with supplemental P treatment. The rostral and medial basal hypothalamic content of oxytocin was significantly higher in macaques with mature gonads. In conclusion, oxytocin neurons do not express nuclear PR and probably do not transcriptionally respond to P. However, gonadal steroids apparently affect the production and release of oxytocin in vivo. Thus, it is possible that oxytocin neurons tranduce the action of P on PRL secretion via stimulatory neurotransmission from another PR-containing neural system.

    Original languageEnglish (US)
    Pages (from-to)945-953
    Number of pages9
    JournalEndocrinology
    Volume134
    Issue number2
    DOIs
    StatePublished - Feb 1994

    Fingerprint

    Oxytocin
    Progesterone Receptors
    Prolactin
    Haplorhini
    Steroids
    Neurons
    Macaca
    Hypothalamic Hormones
    Oxytocin Receptors
    Median Eminence
    Arcuate Nucleus of Hypothalamus
    Preoptic Area
    Paraventricular Hypothalamic Nucleus
    Gonads
    Cytoplasmic and Nuclear Receptors
    Serum
    Synaptic Transmission
    Primates
    Progesterone
    Estrogens

    ASJC Scopus subject areas

    • Endocrinology
    • Endocrinology, Diabetes and Metabolism

    Cite this

    Search for progestin receptors (PR) in prolactin-releasing peptidergic neurons : Oxytocin neurons lack PR, but respond to gonadal steroids in monkeys. / Bethea, Cynthia; Kohama, Steven; Widmann, A. A.

    In: Endocrinology, Vol. 134, No. 2, 02.1994, p. 945-953.

    Research output: Contribution to journalArticle

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    abstract = "Progesterone (P) increases PRL secretion in estrogen (E)-primed primates, but not by a direct action on lactotropes. Oxytocin is one of several hypothalamic hormones that stimulate PRL secretion. This study was conducted to determine whether oxytocin neurons directly mediate the action of P on PRL secretion. Hypothalamic sections from steroid-manipulated macaques were double immunolabeled for oxytocin and progestin receptors (PR). In addition, serum levels of oxytocin were measured in steroid-treated macaques, and hypothalamic levels of oxytocin were measured in monkeys under various physiological conditions. E treatment (28 days) of spayed monkeys caused a significant increase in the number of PR-positive neurons in the preoptic area, ventromedial nucleus, arcuate nucleus, and median eminence. Addition of P to the E treatment for the last 14 of 28 days did not change the number of PR-positive neurons in these areas. The number of PR-positive neurons was low and was unchanged by steroid treatment in the supraoptic and rostral paraventricular nuclei. Oxytocin neurons rarely contained PR regardless of anatomical location, steroid treatment, or fixation protocol. Serum oxytocin levels increased with E treatment and increased further with supplemental P treatment. The rostral and medial basal hypothalamic content of oxytocin was significantly higher in macaques with mature gonads. In conclusion, oxytocin neurons do not express nuclear PR and probably do not transcriptionally respond to P. However, gonadal steroids apparently affect the production and release of oxytocin in vivo. Thus, it is possible that oxytocin neurons tranduce the action of P on PRL secretion via stimulatory neurotransmission from another PR-containing neural system.",
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