Scanning electron microscopy of retinoblastoma

Joseph L. Craft; Nancy L. Robinson; Nancy A. Roth; Daniel M. Albert

doi:10.1016/0014-4835(78)90137-9

Scanning electron microscopy of retinoblastoma

Joseph L. Craft, Nancy L. Robinson, Nancy A. Roth, Daniel M. Albert

Research output: Contribution to journal › Article › peer-review

4 Scopus citations

Abstract

The surface morphology of two continuous cell lines of human retinoblastoma (Y79 and WERI-Rbl) and freshly removed retinoblastoma were studied by scanning electron microscopy (SEM). Commonly observed surface features included microvilli, lamellipodia, filopodia, and zeiotic blebs. A close similarity was seen between the surface morphology of established cell lines of retinoblastoma and that of fresh tumor. Smooth, sphere-like cells were present in both cultured and fresh retinoblastoma. Samples of cultured retinoblastoma cells showed rosettes on light and transmission electron microscopy and the scanning electron microscopic counterparts were observed. The surface characteristics of retinoblastoma were compared to human neuroblastoma and human medulloblastoma grown in tissue culture. Some distinctions in the characteristics were found. No rosettes were seen and the smooth, sphere-like cells were absent. Scanning electron microscopy can be a useful adjunct to light microscopy and transmission electron microscopy (TEM) in the differential diagnosis of small undifferentiated round cell tumors.

Original language	English (US)
Pages (from-to)	519-531
Number of pages	13
Journal	Experimental Eye Research
Volume	27
Issue number	5
DOIs	https://doi.org/10.1016/0014-4835(78)90137-9
State	Published - Nov 1978
Externally published	Yes

Keywords

medulloblastoma
neuroblastoma
retinoblastoma
scanning electron microscopy
surface morphology
undifferentiated round cell tumors

ASJC Scopus subject areas

Ophthalmology
Sensory Systems
Cellular and Molecular Neuroscience

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title = "Scanning electron microscopy of retinoblastoma",

abstract = "The surface morphology of two continuous cell lines of human retinoblastoma (Y79 and WERI-Rbl) and freshly removed retinoblastoma were studied by scanning electron microscopy (SEM). Commonly observed surface features included microvilli, lamellipodia, filopodia, and zeiotic blebs. A close similarity was seen between the surface morphology of established cell lines of retinoblastoma and that of fresh tumor. Smooth, sphere-like cells were present in both cultured and fresh retinoblastoma. Samples of cultured retinoblastoma cells showed rosettes on light and transmission electron microscopy and the scanning electron microscopic counterparts were observed. The surface characteristics of retinoblastoma were compared to human neuroblastoma and human medulloblastoma grown in tissue culture. Some distinctions in the characteristics were found. No rosettes were seen and the smooth, sphere-like cells were absent. Scanning electron microscopy can be a useful adjunct to light microscopy and transmission electron microscopy (TEM) in the differential diagnosis of small undifferentiated round cell tumors.",

keywords = "medulloblastoma, neuroblastoma, retinoblastoma, scanning electron microscopy, surface morphology, undifferentiated round cell tumors",

author = "Craft, {Joseph L.} and Robinson, {Nancy L.} and Roth, {Nancy A.} and Albert, {Daniel M.}",

note = "Funding Information: wish to thank Dr Paul Kornblith of the DepiLrtruerlt of Keurosurpery, General Hospital, for providiug the neuroblastoma and medulloblastoma study was supported in part by National Eye Institute Grant No. EY 191{\textquoteright}7. Copyright: Copyright 2014 Elsevier B.V., All rights reserved.",

year = "1978",

month = nov,

doi = "10.1016/0014-4835(78)90137-9",

language = "English (US)",

volume = "27",

pages = "519--531",

journal = "Experimental Eye Research",

issn = "0014-4835",

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TY - JOUR

T1 - Scanning electron microscopy of retinoblastoma

AU - Craft, Joseph L.

AU - Robinson, Nancy L.

AU - Roth, Nancy A.

AU - Albert, Daniel M.

N1 - Funding Information: wish to thank Dr Paul Kornblith of the DepiLrtruerlt of Keurosurpery, General Hospital, for providiug the neuroblastoma and medulloblastoma study was supported in part by National Eye Institute Grant No. EY 191’7. Copyright: Copyright 2014 Elsevier B.V., All rights reserved.

PY - 1978/11

Y1 - 1978/11

N2 - The surface morphology of two continuous cell lines of human retinoblastoma (Y79 and WERI-Rbl) and freshly removed retinoblastoma were studied by scanning electron microscopy (SEM). Commonly observed surface features included microvilli, lamellipodia, filopodia, and zeiotic blebs. A close similarity was seen between the surface morphology of established cell lines of retinoblastoma and that of fresh tumor. Smooth, sphere-like cells were present in both cultured and fresh retinoblastoma. Samples of cultured retinoblastoma cells showed rosettes on light and transmission electron microscopy and the scanning electron microscopic counterparts were observed. The surface characteristics of retinoblastoma were compared to human neuroblastoma and human medulloblastoma grown in tissue culture. Some distinctions in the characteristics were found. No rosettes were seen and the smooth, sphere-like cells were absent. Scanning electron microscopy can be a useful adjunct to light microscopy and transmission electron microscopy (TEM) in the differential diagnosis of small undifferentiated round cell tumors.

AB - The surface morphology of two continuous cell lines of human retinoblastoma (Y79 and WERI-Rbl) and freshly removed retinoblastoma were studied by scanning electron microscopy (SEM). Commonly observed surface features included microvilli, lamellipodia, filopodia, and zeiotic blebs. A close similarity was seen between the surface morphology of established cell lines of retinoblastoma and that of fresh tumor. Smooth, sphere-like cells were present in both cultured and fresh retinoblastoma. Samples of cultured retinoblastoma cells showed rosettes on light and transmission electron microscopy and the scanning electron microscopic counterparts were observed. The surface characteristics of retinoblastoma were compared to human neuroblastoma and human medulloblastoma grown in tissue culture. Some distinctions in the characteristics were found. No rosettes were seen and the smooth, sphere-like cells were absent. Scanning electron microscopy can be a useful adjunct to light microscopy and transmission electron microscopy (TEM) in the differential diagnosis of small undifferentiated round cell tumors.

KW - medulloblastoma

KW - neuroblastoma

KW - retinoblastoma

KW - scanning electron microscopy

KW - surface morphology

KW - undifferentiated round cell tumors

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JO - Experimental Eye Research

JF - Experimental Eye Research

SN - 0014-4835

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Scanning electron microscopy of retinoblastoma

Abstract

Keywords

ASJC Scopus subject areas

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