Abstract
The surface morphology of two continuous cell lines of human retinoblastoma (Y79 and WERI-Rbl) and freshly removed retinoblastoma were studied by scanning electron microscopy (SEM). Commonly observed surface features included microvilli, lamellipodia, filopodia, and zeiotic blebs. A close similarity was seen between the surface morphology of established cell lines of retinoblastoma and that of fresh tumor. Smooth, sphere-like cells were present in both cultured and fresh retinoblastoma. Samples of cultured retinoblastoma cells showed rosettes on light and transmission electron microscopy and the scanning electron microscopic counterparts were observed. The surface characteristics of retinoblastoma were compared to human neuroblastoma and human medulloblastoma grown in tissue culture. Some distinctions in the characteristics were found. No rosettes were seen and the smooth, sphere-like cells were absent. Scanning electron microscopy can be a useful adjunct to light microscopy and transmission electron microscopy (TEM) in the differential diagnosis of small undifferentiated round cell tumors.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 519-531 |
| Number of pages | 13 |
| Journal | Experimental Eye Research |
| Volume | 27 |
| Issue number | 5 |
| DOIs | |
| State | Published - Jan 1 1978 |
| Externally published | Yes |
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Keywords
- medulloblastoma
- neuroblastoma
- retinoblastoma
- scanning electron microscopy
- surface morphology
- undifferentiated round cell tumors
ASJC Scopus subject areas
- Ophthalmology
- Sensory Systems
- Cellular and Molecular Neuroscience
Cite this
Scanning electron microscopy of retinoblastoma. / Craft, Joseph L.; Robinson, Nancy L.; Roth, Nancy A.; Albert, Daniel.
In: Experimental Eye Research, Vol. 27, No. 5, 01.01.1978, p. 519-531.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Scanning electron microscopy of retinoblastoma
AU - Craft, Joseph L.
AU - Robinson, Nancy L.
AU - Roth, Nancy A.
AU - Albert, Daniel
PY - 1978/1/1
Y1 - 1978/1/1
N2 - The surface morphology of two continuous cell lines of human retinoblastoma (Y79 and WERI-Rbl) and freshly removed retinoblastoma were studied by scanning electron microscopy (SEM). Commonly observed surface features included microvilli, lamellipodia, filopodia, and zeiotic blebs. A close similarity was seen between the surface morphology of established cell lines of retinoblastoma and that of fresh tumor. Smooth, sphere-like cells were present in both cultured and fresh retinoblastoma. Samples of cultured retinoblastoma cells showed rosettes on light and transmission electron microscopy and the scanning electron microscopic counterparts were observed. The surface characteristics of retinoblastoma were compared to human neuroblastoma and human medulloblastoma grown in tissue culture. Some distinctions in the characteristics were found. No rosettes were seen and the smooth, sphere-like cells were absent. Scanning electron microscopy can be a useful adjunct to light microscopy and transmission electron microscopy (TEM) in the differential diagnosis of small undifferentiated round cell tumors.
AB - The surface morphology of two continuous cell lines of human retinoblastoma (Y79 and WERI-Rbl) and freshly removed retinoblastoma were studied by scanning electron microscopy (SEM). Commonly observed surface features included microvilli, lamellipodia, filopodia, and zeiotic blebs. A close similarity was seen between the surface morphology of established cell lines of retinoblastoma and that of fresh tumor. Smooth, sphere-like cells were present in both cultured and fresh retinoblastoma. Samples of cultured retinoblastoma cells showed rosettes on light and transmission electron microscopy and the scanning electron microscopic counterparts were observed. The surface characteristics of retinoblastoma were compared to human neuroblastoma and human medulloblastoma grown in tissue culture. Some distinctions in the characteristics were found. No rosettes were seen and the smooth, sphere-like cells were absent. Scanning electron microscopy can be a useful adjunct to light microscopy and transmission electron microscopy (TEM) in the differential diagnosis of small undifferentiated round cell tumors.
KW - medulloblastoma
KW - neuroblastoma
KW - retinoblastoma
KW - scanning electron microscopy
KW - surface morphology
KW - undifferentiated round cell tumors
UR - http://www.scopus.com/inward/record.url?scp=0018181443&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0018181443&partnerID=8YFLogxK
U2 - 10.1016/0014-4835(78)90137-9
DO - 10.1016/0014-4835(78)90137-9
M3 - Article
C2 - 720426
AN - SCOPUS:0018181443
VL - 27
SP - 519
EP - 531
JO - Experimental Eye Research
JF - Experimental Eye Research
SN - 0014-4835
IS - 5
ER -