Scanning electron microscopy of isolated peripheral nerve fibres - Normal surface structure and alterations proximal to neuromas

Peter S. Spencer, A. R. Lieberman

Research output: Contribution to journalArticle

36 Scopus citations

Abstract

The surface morphology of normal myelinated nerve fibres prepared in different ways for scanning electron microscopy has been studied and compared with the surface features of similar fibres undergoing retrograde changes. Nodes of Ranvier, paranodal specializations, artefactual fractures of the myelin, and the endoneurial collagen sheaths are described. A regular pattern of elevations, usually with a pitted or depressed surface seen on normal myelinated fibres after certain preparative procedures are thought to be artefacts produced during preparation and to be related to the neurokeratin network. Alterations in the surface structure of fibres central to long-standing nerve transections include irregular protuberances, serial surface corrugations and large swellings, all associated with demyelination. Fibres that have undergone retrograde degeneration consist of endoneurial tubes with focal swellings occupied by macrophages or myelin debris, together with fine unmyelinated and small myelinated regenerating axons. Strict centrifugal progression of myelination of regenerating axons was not observed.

Original languageEnglish (US)
Pages (from-to)534-551
Number of pages18
JournalZeitschrift für Zellforschung und Mikroskopische Anatomie
Volume119
Issue number4
DOIs
StatePublished - Dec 1971

Keywords

  • Demyelination
  • Isolated nerve fibres
  • Neurokeratin
  • Nodes of Ranvier
  • Scanning electron microscopy

ASJC Scopus subject areas

  • Pathology and Forensic Medicine
  • Histology
  • Cell Biology

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