TY - JOUR
T1 - SAP97 concentrates at the postsynaptic density in cerebral cortex
AU - Valtschanoff, Juli G.
AU - Burette, Alain
AU - Davare, Monika A.
AU - Soren Leonard, A.
AU - Hell, Johannes W.
AU - Weinberg, Richard J.
PY - 2000
Y1 - 2000
N2 - SAP97, a PDZ-containing protein, is reported to concentrate in axon terminals, where its function remains unknown. Using highly specific new antibodies, we show that SAP97 in rat cerebral cortex is associated with heteromeric AMPA receptors via a selective biochemical interaction between SAP97 and the GluR1 subunit. Using light and electron microscopic immunocytochemistry, we demonstrate cellular and synaptic colocalization of SAP97 and GluR1, and show that SAP97 concentrates at synapses that contain GluR1 but not necessarily GluR2 or GluR3. Using quantitative postembedding immunogold electron microscopy, we find that SAP97 is at highest concentration within the postsynaptic density of asymmetric synapses. These data suggest that SAP97 may help to anchor GluR1-containing AMPA receptors at the synapse. As a multifunctional scaffolding protein, SAP97 may organize components of AMPA-related intracellular signalling pathways, including those associated with calcium-permeable homomeric GluR1 channels.
AB - SAP97, a PDZ-containing protein, is reported to concentrate in axon terminals, where its function remains unknown. Using highly specific new antibodies, we show that SAP97 in rat cerebral cortex is associated with heteromeric AMPA receptors via a selective biochemical interaction between SAP97 and the GluR1 subunit. Using light and electron microscopic immunocytochemistry, we demonstrate cellular and synaptic colocalization of SAP97 and GluR1, and show that SAP97 concentrates at synapses that contain GluR1 but not necessarily GluR2 or GluR3. Using quantitative postembedding immunogold electron microscopy, we find that SAP97 is at highest concentration within the postsynaptic density of asymmetric synapses. These data suggest that SAP97 may help to anchor GluR1-containing AMPA receptors at the synapse. As a multifunctional scaffolding protein, SAP97 may organize components of AMPA-related intracellular signalling pathways, including those associated with calcium-permeable homomeric GluR1 channels.
KW - AMPA receptors, GluR1, Immunocytochemistry, PDZ, Rat
KW - Scaffolding proteins
UR - http://www.scopus.com/inward/record.url?scp=0033792684&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0033792684&partnerID=8YFLogxK
U2 - 10.1046/j.1460-9568.2000.00256.x
DO - 10.1046/j.1460-9568.2000.00256.x
M3 - Article
C2 - 11029631
AN - SCOPUS:0033792684
SN - 0953-816X
VL - 12
SP - 3605
EP - 3614
JO - European Journal of Neuroscience
JF - European Journal of Neuroscience
IS - 10
ER -