SAP97 concentrates at the postsynaptic density in cerebral cortex

Juli G. Valtschanoff, Alain Burette, Monika A. Davare, A. Soren Leonard, Johannes W. Hell, Richard J. Weinberg

Research output: Contribution to journalArticle

84 Scopus citations

Abstract

SAP97, a PDZ-containing protein, is reported to concentrate in axon terminals, where its function remains unknown. Using highly specific new antibodies, we show that SAP97 in rat cerebral cortex is associated with heteromeric AMPA receptors via a selective biochemical interaction between SAP97 and the GluR1 subunit. Using light and electron microscopic immunocytochemistry, we demonstrate cellular and synaptic colocalization of SAP97 and GluR1, and show that SAP97 concentrates at synapses that contain GluR1 but not necessarily GluR2 or GluR3. Using quantitative postembedding immunogold electron microscopy, we find that SAP97 is at highest concentration within the postsynaptic density of asymmetric synapses. These data suggest that SAP97 may help to anchor GluR1-containing AMPA receptors at the synapse. As a multifunctional scaffolding protein, SAP97 may organize components of AMPA-related intracellular signalling pathways, including those associated with calcium-permeable homomeric GluR1 channels.

Original languageEnglish (US)
Pages (from-to)3605-3614
Number of pages10
JournalEuropean Journal of Neuroscience
Volume12
Issue number10
DOIs
StatePublished - Nov 7 2000

Keywords

  • AMPA receptors, GluR1, Immunocytochemistry, PDZ, Rat
  • Scaffolding proteins

ASJC Scopus subject areas

  • Neuroscience(all)

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    Valtschanoff, J. G., Burette, A., Davare, M. A., Soren Leonard, A., Hell, J. W., & Weinberg, R. J. (2000). SAP97 concentrates at the postsynaptic density in cerebral cortex. European Journal of Neuroscience, 12(10), 3605-3614. https://doi.org/10.1046/j.1460-9568.2000.00256.x