Salmonella typhimurium proliferates and establishes a persistent infection in the intestine of Caenorhabditis elegans

Alejandro Aballay, Peter Yorgey, Frederick M. Ausubel

Research output: Contribution to journalArticle

233 Citations (Scopus)

Abstract

Genetic analysis of host-pathogen interactions has been hampered by the lack of genetically tractable models of such interactions. We showed previously that the human opportunistic pathogen Pseudomonas aeruginosa kills Caenorhabditis elegans, that P. aeruginosa and C. elegans genes can be identified that affect this killing, and that most of these P. aeruginosa genes are also important for mammalian pathogenesis. Here, we show that Salmonella typhimurium as well as other Salmonella enterica serovars including S. enteritidis and S. dublin can also kill C. elegans. When C. elegans is placed on a lawn of S. typhimurium, the bacteria accumulate in the lumen of the worm intestine and the nematodes die over the course of several days. This killing requires contact with live bacterial cells. The worms die with similar kinetics when placed on a lawn of S. typhimurium for a relatively short time (3-5 hours) before transfer to a lawn of E. coli. After the transfer to E. coli, a high titer of S. typhimurium persists in the C. elegans intestinal lumen for the rest of the worms' life. Furthermore, feeding for 5 hours on a 1:1000 mixture of S. typhimurium and E. coli followed by transfer to 100% E. coli, also led to death after several days. This killing correlated with an increase in the titer of S. typhimurium in the C. elegans lumen, which reached 10,000 bacteria per worm. These data indicate that, in contrast to P. aeruginosa, a small inoculum of S. typhimurium can proliferate in the C. elegans intestine and establish a persistent infection. S. typhimurium mutated in the PhoP/PhoQ signal transduction system caused significantly less killing of C. elegans.

Original languageEnglish (US)
Pages (from-to)1539-1542
Number of pages4
JournalCurrent Biology
Volume10
Issue number23
DOIs
StatePublished - Nov 30 2000
Externally publishedYes

Fingerprint

Salmonella
Caenorhabditis elegans
Salmonella typhimurium
Salmonella Typhimurium
Escherichia coli
Intestines
intestines
Pathogens
Infection
Pseudomonas aeruginosa
infection
Bacteria
Genes
Signal transduction
Salmonella Dublin
Host-Pathogen Interactions
Kinetics
Salmonella enterica
host-pathogen relationships
bacteria

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)

Cite this

Salmonella typhimurium proliferates and establishes a persistent infection in the intestine of Caenorhabditis elegans. / Aballay, Alejandro; Yorgey, Peter; Ausubel, Frederick M.

In: Current Biology, Vol. 10, No. 23, 30.11.2000, p. 1539-1542.

Research output: Contribution to journalArticle

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