Salmonella typhimurium loci involved in survival within macrophages

A. J. Baumler, J. G. Kusters, I. Stojiljkovic, Fred Heffron

    Research output: Contribution to journalArticle

    175 Citations (Scopus)

    Abstract

    A set of Tn10 mutants of Salmonella typhimurium which have a diminished capacity to survive in murine macrophages and decreased virulence in mice has been described previously. In this study, we characterized 30 of these mutants and determined map locations of Tn10 insertions for 23 of these strains. In addition, short fragments of transposon-flanking DNA were cloned, and the nucleotide sequence was determined for 23 mutants. Seven mutants carried transposon insertions in known genes, representing six loci: htrA, prc, purD, fliD, nagA, and smpB. The possible roles of these genes in Salmonella virulence are discussed. One insertion was found to be in an unknown gene which shared homology with the open reading frames Bv' and Bv located in the pin inversion system of Shigella boydii. In one mutant, Tn10 was found to be inserted in a gene with significant homology to adhE of Escherichia coli and Clostridium acetobutylicum. The map location and degree of homology indicate that the Salmonella gene encodes a related, but different, dehydrogenase. In 14 of the mutants analyzed, Tn10 was inserted into genes which had no significant homologies to entries in the DNA and protein data bases. In conclusion, 16 insertions define loci, termed ims for impaired macrophage survival, which have not yet been described in S. typhimurium but have been shown previously to be necessary for full virulence in mice. Although most ims loci are distributed randomly throughout the genome, a cluster was found between 75 and 78 min on the Salmonella chromosome.

    Original languageEnglish (US)
    Pages (from-to)1623-1630
    Number of pages8
    JournalInfection and Immunity
    Volume62
    Issue number5
    StatePublished - 1994

    Fingerprint

    Salmonella typhimurium
    Macrophages
    Salmonella
    Genes
    Virulence
    Shigella boydii
    Clostridium acetobutylicum
    DNA Transposable Elements
    Open Reading Frames
    Oxidoreductases
    Chromosomes
    Genome
    Databases
    Escherichia coli
    DNA
    Proteins

    ASJC Scopus subject areas

    • Immunology

    Cite this

    Baumler, A. J., Kusters, J. G., Stojiljkovic, I., & Heffron, F. (1994). Salmonella typhimurium loci involved in survival within macrophages. Infection and Immunity, 62(5), 1623-1630.

    Salmonella typhimurium loci involved in survival within macrophages. / Baumler, A. J.; Kusters, J. G.; Stojiljkovic, I.; Heffron, Fred.

    In: Infection and Immunity, Vol. 62, No. 5, 1994, p. 1623-1630.

    Research output: Contribution to journalArticle

    Baumler, AJ, Kusters, JG, Stojiljkovic, I & Heffron, F 1994, 'Salmonella typhimurium loci involved in survival within macrophages', Infection and Immunity, vol. 62, no. 5, pp. 1623-1630.
    Baumler AJ, Kusters JG, Stojiljkovic I, Heffron F. Salmonella typhimurium loci involved in survival within macrophages. Infection and Immunity. 1994;62(5):1623-1630.
    Baumler, A. J. ; Kusters, J. G. ; Stojiljkovic, I. ; Heffron, Fred. / Salmonella typhimurium loci involved in survival within macrophages. In: Infection and Immunity. 1994 ; Vol. 62, No. 5. pp. 1623-1630.
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