Salmonella SsrB activates a global regulon of horizontally acquired genes

Micah J. Worley, Katherine H L Ching, Fred Heffron

    Research output: Contribution to journalArticle

    152 Citations (Scopus)

    Abstract

    Salmonella enterica is a bacterial pathogen of humans that can proliferate within epithelial cells as well as professional phagocytes of the immune system. This ability requires an S. enterica specific locus termed Salmonella pathogenicity island 2 (SPI-2). SPI-2 encodes a type III secretion system that injects effecters encoded within the island into host cell cytosol to promote virulence. SsrAB is a two-component regulator encoded within SPI-2 that was assumed to activate SPI-2 genes exclusively. Here, it is shown that SsrB in fact activates a global regulon. At least 10 genes outside SPI-2 are SsrB regulated within epithelial and macrophage cells. Nine of these 10 SsrB-regulated genes outside SPI-2 reside within previously undescribed regions of the Salmonella genome. Most share no sequence homology with current database entries. However, one is remarkably homologous to human glucosyl ceramidase, an enzyme involved in the ceramide signalling pathway. The SsrB regulon is modulated by the two-component regulatory systems PhoP/PhoQ and OmpR/ EnvZ, and is upregulated in the intracellular microenvironment.

    Original languageEnglish (US)
    Pages (from-to)749-761
    Number of pages13
    JournalMolecular Microbiology
    Volume36
    Issue number3
    DOIs
    StatePublished - 2000

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    Regulon
    Genomic Islands
    Salmonella
    Genes
    Salmonella enterica
    Epithelial Cells
    Glucosylceramidase
    Ceramides
    Phagocytes
    Sequence Homology
    Islands
    Cytosol
    Virulence
    Immune System
    Macrophages
    Genome
    Databases
    Enzymes

    ASJC Scopus subject areas

    • Molecular Biology
    • Microbiology

    Cite this

    Salmonella SsrB activates a global regulon of horizontally acquired genes. / Worley, Micah J.; Ching, Katherine H L; Heffron, Fred.

    In: Molecular Microbiology, Vol. 36, No. 3, 2000, p. 749-761.

    Research output: Contribution to journalArticle

    Worley, Micah J. ; Ching, Katherine H L ; Heffron, Fred. / Salmonella SsrB activates a global regulon of horizontally acquired genes. In: Molecular Microbiology. 2000 ; Vol. 36, No. 3. pp. 749-761.
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