Salicylate-independent lesion formation in Arabidopsis lsd mutants

Michelle D. Hunt, Terrence P. Delaney, Robert A. Dietrich, Kristianna Weymann, Jeffery L. Dangl, John A. Ryals

Research output: Contribution to journalArticle

65 Citations (Scopus)

Abstract

In many interactions of plants with pathogens, the primary host defense reaction is accompanied by plant cell death at the site of infection. The resulting lesions are correlated with the establishment of an inducible resistance in plants called systemic acquired resistance (SAR), for which salicylic acid (SA) accumulation is a critical signaling event in Arabidopsis and tobacco. In Arabidopsis, the lesions simulating disease (lsd) mutants spontaneously develop lesions in the absence of pathogen infection. Furthermore, lsd mutants express SAR marker genes when lesions are present and are resistant to the same spectrum of pathogens as plants activated for SAR by necrogenic pathogen infection. To assess the epistatic relationship between SA accumulation and cell death, transgenic Arabidopsis unable to accumulate SA due to the expression of the salicylate hydroxylase (nahG) gene were used in crosses with the dominant mutants lsd2 or lsd4. Progeny from the crosses were inhibited for SAR gene expression and disease resistance. However, these progeny retained the spontaneous cell death phenotype similar to siblings not expressing nahG. Because lesions form in the absence of SA accumulation for lsd2 and lsd4, a model is suggested in which lesion formation in these two mutants is determined prior to SA accumulation in SAR signal transduction. By contrast, the loss of SAR gene expression and disease resistance in nahG-expressing lsd mutants indicates that these traits are dependent upon SA accumulation in the SAR signal transduction pathway.

Original languageEnglish (US)
Pages (from-to)531-536
Number of pages6
JournalMolecular Plant-Microbe Interactions
Volume10
Issue number5
StatePublished - Jul 1997
Externally publishedYes

Fingerprint

salicylates
lesions (plant)
Salicylic Acid
Salicylates
systemic acquired resistance
Arabidopsis
salicylic acid
mutants
Disease Resistance
Cell Death
salicylate 1-monooxygenase
cell death
pathogens
Signal Transduction
Infection
disease resistance
signal transduction
Gene Expression
Plant Cells
infection

Keywords

  • Autofluorescence
  • Callose
  • Feedback amplification loop
  • Peronospora parasitica isolate Noco
  • PR gene expression

ASJC Scopus subject areas

  • Physiology
  • Agronomy and Crop Science

Cite this

Hunt, M. D., Delaney, T. P., Dietrich, R. A., Weymann, K., Dangl, J. L., & Ryals, J. A. (1997). Salicylate-independent lesion formation in Arabidopsis lsd mutants. Molecular Plant-Microbe Interactions, 10(5), 531-536.

Salicylate-independent lesion formation in Arabidopsis lsd mutants. / Hunt, Michelle D.; Delaney, Terrence P.; Dietrich, Robert A.; Weymann, Kristianna; Dangl, Jeffery L.; Ryals, John A.

In: Molecular Plant-Microbe Interactions, Vol. 10, No. 5, 07.1997, p. 531-536.

Research output: Contribution to journalArticle

Hunt, MD, Delaney, TP, Dietrich, RA, Weymann, K, Dangl, JL & Ryals, JA 1997, 'Salicylate-independent lesion formation in Arabidopsis lsd mutants', Molecular Plant-Microbe Interactions, vol. 10, no. 5, pp. 531-536.
Hunt MD, Delaney TP, Dietrich RA, Weymann K, Dangl JL, Ryals JA. Salicylate-independent lesion formation in Arabidopsis lsd mutants. Molecular Plant-Microbe Interactions. 1997 Jul;10(5):531-536.
Hunt, Michelle D. ; Delaney, Terrence P. ; Dietrich, Robert A. ; Weymann, Kristianna ; Dangl, Jeffery L. ; Ryals, John A. / Salicylate-independent lesion formation in Arabidopsis lsd mutants. In: Molecular Plant-Microbe Interactions. 1997 ; Vol. 10, No. 5. pp. 531-536.
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