TY - JOUR
T1 - S-Nitrosoglutathione Reductase (GSNOR) Deficiency Results in Secondary Hypogonadism
AU - Masterson, Thomas A.
AU - Arora, Himanshu
AU - Kulandavelu, Shathiyah
AU - Carroll, Rona S.
AU - Kaiser, Ursula B.
AU - Gultekin, Sakir H.
AU - Hare, Joshua M.
AU - Ramasamy, Ranjith
N1 - Publisher Copyright:
© 2018 International Society for Sexual Medicine
PY - 2018/5
Y1 - 2018/5
N2 - Background: Excess reactive oxygen species and reactive nitrogen species are implicated in male infertility and impaired spermatogenesis. Aim: To investigate the effect of excess reactive nitrogen species and nitrosative stress on testicular function and the hypothalamic-pituitary-gonadal axis using the S-nitrosoglutathione reductase-null (Gsnor−/−) mouse model. Methods: Testis size, pup number, and epididymal sperm concentration and motility of Gsnor−/− mice were compared with those of age-matched wild-type (WT) mice. Reproductive hormones testosterone (T), luteinizing hormone (LH), and follicle-stimulating hormone were compared in Gsnor−/− and WT mice. Immunofluorescence for Gsnor−/− and WT testis was performed for 3β-hydroxysteroid dehydrogenase and luteinizing hormone receptor (LHR) and compared. Human chorionic gonadotropin and gonadotropin-releasing hormone stimulation tests were performed to assess and compare testicular and pituitary functions of Gsnor−/− and WT mice. Outcomes: Evaluation of fertility and reproductive hormones in Gsnor−/− vs WT mice. Response of Gsnor−/− and WT mice to human chorionic gonadotropin and gonadotropin-releasing hormone to evaluate LH and T production. Results: Gsnor−/− mice had smaller litters (4.2 vs 8.0 pups per litter; P <.01), smaller testes (0.08 vs 0.09 g; P <.01), and decreased epididymal sperm concentration (69 vs 98 × 106; P <.05) and motility (39% vs 65%; P <.05) compared with WT mice. Serum T (44.8 vs 292.2 ng/dL; P <.05) and LH (0.03 vs 0.74 ng/mL; P =.04) were lower in Gsnor−/− than in WT mice despite similar follicle-stimulating hormone levels (63.98 vs 77.93 ng/mL; P =.20). Immunofluorescence of Gsnor−/− and WT testes showed similar staining of 3β-hydroxysteroid dehydrogenase and LHR. Human chorionic gonadotropin stimulation of Gsnor−/− mice increased serum T (>1,680 vs >1,680 ng/dL) and gonadotropin-releasing hormone stimulation increased serum LH (6.3 vs 8.9 ng/mL; P =.20) similar to WT mice. Clinical Translation: These findings provide novel insight to a possible mechanism of secondary hypogonadism from increased reactive nitrogen species and excess nitrosative stress. Strengths and Limitations: Limitations of this study are its small samples and variability in hormone levels. Conclusion: Deficiency of S-nitrosoglutathione reductase results in secondary hypogonadism, suggesting that excess nitrosative stress can affect LH production from the pituitary gland. Masterson TA, Arora H, Kulandavelu S, et al. S-Nitrosoglutathione Reductase (GSNOR) Deficiency Results in Secondary Hypogonadism. J Sex Med 2018;15:654–661.
AB - Background: Excess reactive oxygen species and reactive nitrogen species are implicated in male infertility and impaired spermatogenesis. Aim: To investigate the effect of excess reactive nitrogen species and nitrosative stress on testicular function and the hypothalamic-pituitary-gonadal axis using the S-nitrosoglutathione reductase-null (Gsnor−/−) mouse model. Methods: Testis size, pup number, and epididymal sperm concentration and motility of Gsnor−/− mice were compared with those of age-matched wild-type (WT) mice. Reproductive hormones testosterone (T), luteinizing hormone (LH), and follicle-stimulating hormone were compared in Gsnor−/− and WT mice. Immunofluorescence for Gsnor−/− and WT testis was performed for 3β-hydroxysteroid dehydrogenase and luteinizing hormone receptor (LHR) and compared. Human chorionic gonadotropin and gonadotropin-releasing hormone stimulation tests were performed to assess and compare testicular and pituitary functions of Gsnor−/− and WT mice. Outcomes: Evaluation of fertility and reproductive hormones in Gsnor−/− vs WT mice. Response of Gsnor−/− and WT mice to human chorionic gonadotropin and gonadotropin-releasing hormone to evaluate LH and T production. Results: Gsnor−/− mice had smaller litters (4.2 vs 8.0 pups per litter; P <.01), smaller testes (0.08 vs 0.09 g; P <.01), and decreased epididymal sperm concentration (69 vs 98 × 106; P <.05) and motility (39% vs 65%; P <.05) compared with WT mice. Serum T (44.8 vs 292.2 ng/dL; P <.05) and LH (0.03 vs 0.74 ng/mL; P =.04) were lower in Gsnor−/− than in WT mice despite similar follicle-stimulating hormone levels (63.98 vs 77.93 ng/mL; P =.20). Immunofluorescence of Gsnor−/− and WT testes showed similar staining of 3β-hydroxysteroid dehydrogenase and LHR. Human chorionic gonadotropin stimulation of Gsnor−/− mice increased serum T (>1,680 vs >1,680 ng/dL) and gonadotropin-releasing hormone stimulation increased serum LH (6.3 vs 8.9 ng/mL; P =.20) similar to WT mice. Clinical Translation: These findings provide novel insight to a possible mechanism of secondary hypogonadism from increased reactive nitrogen species and excess nitrosative stress. Strengths and Limitations: Limitations of this study are its small samples and variability in hormone levels. Conclusion: Deficiency of S-nitrosoglutathione reductase results in secondary hypogonadism, suggesting that excess nitrosative stress can affect LH production from the pituitary gland. Masterson TA, Arora H, Kulandavelu S, et al. S-Nitrosoglutathione Reductase (GSNOR) Deficiency Results in Secondary Hypogonadism. J Sex Med 2018;15:654–661.
KW - Nitrosative Stress
KW - Reactive Nitrogen Species
KW - Secondary Hypogonadism
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U2 - 10.1016/j.jsxm.2018.03.002
DO - 10.1016/j.jsxm.2018.03.002
M3 - Article
AN - SCOPUS:85044669752
SN - 1743-6095
VL - 15
SP - 654
EP - 661
JO - Journal of Sexual Medicine
JF - Journal of Sexual Medicine
IS - 5
ER -