RRE-dependent HIV-1 Env RNA effects on Gag protein expression, assembly and release

Claudia Lopez, Rachel Sloan, Isabel Cylinder, Susan L. Kozak, David Kabat, Eric Barklis

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

The HIV-1 Gag proteins are translated from the full-length HIV-1 viral RNA (vRNA), whereas the envelope (Env) protein is translated from incompletely spliced Env mRNAs. Nuclear export of vRNAs and Env mRNAs is mediated by the Rev accessory protein which binds to the rev-responsive element (RRE) present on these RNAs. Evidence has shown there is a direct or indirect interaction between the Gag protein, and the cytoplasmic tail (CT) of the Env protein. Our current work shows that env gene expression impacts HIV-1 Gag expression and function in two ways. At the protein level, full-length Env expression altered Gag protein expression, while Env CT-deletion proteins did not. At the RNA level, RRE-containing Env mRNA expression reduced Gag expression, processing, and virus particle release from cells. Our results support models in which Gag is influenced by the Env CT, and Env mRNAs compete with vRNAs for nuclear export.

Original languageEnglish (US)
Pages (from-to)126-134
Number of pages9
JournalVirology
Volume462-463
Issue number1
DOIs
StatePublished - 2014

Fingerprint

env Genes
gag Gene Products
HIV-1
RNA
Messenger RNA
Cell Nucleus Active Transport
Proteins
rev Gene Products
Virus Release
Nuclear Envelope
Viral RNA
Virion
Gene Expression

Keywords

  • Env
  • Gag
  • HIV-1
  • RNA export
  • RRE

ASJC Scopus subject areas

  • Virology

Cite this

RRE-dependent HIV-1 Env RNA effects on Gag protein expression, assembly and release. / Lopez, Claudia; Sloan, Rachel; Cylinder, Isabel; Kozak, Susan L.; Kabat, David; Barklis, Eric.

In: Virology, Vol. 462-463, No. 1, 2014, p. 126-134.

Research output: Contribution to journalArticle

Lopez, Claudia ; Sloan, Rachel ; Cylinder, Isabel ; Kozak, Susan L. ; Kabat, David ; Barklis, Eric. / RRE-dependent HIV-1 Env RNA effects on Gag protein expression, assembly and release. In: Virology. 2014 ; Vol. 462-463, No. 1. pp. 126-134.
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