Routine aerobic terminal subculturing of blood cultures in a cancer hospital

T. E. Kiehn, Brian Wong, F. F. Edwards, D. Armstrong

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Routine terminal aerobic subcultures of macroscopically negative blood culture bottles were evaluated during a 15-month period when 30,000 blood cultures were processed. Each blood culture set consisted of a vented and an unvented 50-ml broth bottle. Forth-eight pathogens and 47 contaminants were isolated only from terminal subcultures. Twenty-two of the significant isolates were yeasts (usually recovered from vented bottles), and 10 were Pseudomonas aeruginosa (usually recovered from unvented bottles). Blood cultures that were positive by terminal subculture provided clinically relevant information in many cases, whether other blood cultures were positive or not. Microbiology laboratories, particularly those in hospitals where yeasts and P. aeruginosa are commonly isolated from blood specimens, should evaluate carefully the need for terminal subcultures of blood culture bottles before abandoning their use.

Original languageEnglish (US)
Pages (from-to)885-889
Number of pages5
JournalJournal of Clinical Microbiology
Volume18
Issue number4
StatePublished - 1983
Externally publishedYes

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Cancer Care Facilities
Pseudomonas aeruginosa
Yeasts
Microbiology
Blood Culture

ASJC Scopus subject areas

  • Microbiology
  • Microbiology (medical)

Cite this

Kiehn, T. E., Wong, B., Edwards, F. F., & Armstrong, D. (1983). Routine aerobic terminal subculturing of blood cultures in a cancer hospital. Journal of Clinical Microbiology, 18(4), 885-889.

Routine aerobic terminal subculturing of blood cultures in a cancer hospital. / Kiehn, T. E.; Wong, Brian; Edwards, F. F.; Armstrong, D.

In: Journal of Clinical Microbiology, Vol. 18, No. 4, 1983, p. 885-889.

Research output: Contribution to journalArticle

Kiehn, TE, Wong, B, Edwards, FF & Armstrong, D 1983, 'Routine aerobic terminal subculturing of blood cultures in a cancer hospital', Journal of Clinical Microbiology, vol. 18, no. 4, pp. 885-889.
Kiehn, T. E. ; Wong, Brian ; Edwards, F. F. ; Armstrong, D. / Routine aerobic terminal subculturing of blood cultures in a cancer hospital. In: Journal of Clinical Microbiology. 1983 ; Vol. 18, No. 4. pp. 885-889.
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