Rotenone enhances N-methyl-D-aspartate currents by activating a tyrosine kinase in rat dopamine neurons

Yan Na Wu, Giuseppina Martella, Steven W. Johnson

Research output: Contribution to journalArticle

9 Scopus citations

Abstract

Our previous work showed that the pesticide rotenone increases the amplitude of inward currents evoked by N-methyl-D-aspartate (NMDA) in substantia nigra dopamine neurons. Using patch pipettes to record whole-cell currents in rat brain slices, we report that the rotenone-induced potentiation of NMDA current is blocked by the tyrosine kinase inhibitors genistein and PP1. This action of rotenone is mimicked by H2O2, which is also blocked by genistein. Our results suggest that the rotenone-dependent increase in NMDA current is mediated by release of reactive oxygen species that activates a protein tyrosine kinase.

Original languageEnglish (US)
Pages (from-to)1813-1816
Number of pages4
JournalNeuroReport
Volume18
Issue number17
DOIs
StatePublished - Nov 1 2007

Keywords

  • Brain slice
  • Excitotoxicity
  • Hydrogen peroxide
  • N-methyl-D-aspartate
  • Patch clamp
  • Reactive oxygen species
  • Rotenone
  • Substantia nigra
  • Tyrosine kinase

ASJC Scopus subject areas

  • Neuroscience(all)

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