Rosiglitazone reduces serum homocysteine levels, smooth muscle proliferation, and intimal hyperplasia in Sprague-Dawley rats fed a high methionine diet

Subramanyam N. Murthy, Demian F. Obregon, Natasha Chattergoon, Neil A. Fonseca, Debasis Mondal, Jeffrey B. Dunne, Jose G. Diez, James R. Jeter, Philip J. Kadowitz, Krishna C. Agrawal, Dennis B. McNamara, Vivian A. Fonseca

Research output: Contribution to journalArticle

39 Citations (Scopus)

Abstract

Homocysteine (Hcy) is a metabolite of the essential amino acid methionine. Hyperhomocysteinemia is associated with vascular disease, particularly carotid stenosis. Rosiglitazone, a ligand of the peroxisome proliferator-activated receptor γ, attenuates balloon catheter-induced carotid intimal hyperplasia in type 2 diabetic rats. We studied 4 groups (n = 7 per group) of adult female Sprague-Dawley rats fed (a) powdered laboratory chow (control), (b) control diet with rosiglitazone (3.0 mg/kg/d), (c) diet containing 1.0% l-methionine, and (d) diet containing methionine and rosiglitazone. After 1 week on high methionine diet, the rats were administered an aqueous preparation of rosiglitazone by oral gavage. One week after initiation of rosiglitazone, balloon catheter injury of the carotid artery was carried out using established methods, and the animals continued on their respective dietary and drug regimens for another 21 days. At the end of the experimental period, blood samples were collected, and carotid arteries and liver were harvested. Serum Hcy increased significantly on methionine diet compared with controls (28.9 ± 3.2 vs 6.3 ± 0.04 μmol/L). Development of intimal hyperplasia was 4-fold higher in methionine-fed rats; this augmentation was significantly reduced (P

Original languageEnglish (US)
Pages (from-to)645-652
Number of pages8
JournalMetabolism: Clinical and Experimental
Volume54
Issue number5
DOIs
StatePublished - May 2005
Externally publishedYes

Fingerprint

rosiglitazone
Tunica Intima
Homocysteine
Methionine
Hyperplasia
Smooth Muscle
Sprague Dawley Rats
Diet
Serum
Catheters
Carotid Artery Injuries
Dilatation and Curettage
Hyperhomocysteinemia
Peroxisome Proliferator-Activated Receptors
Essential Amino Acids
Carotid Stenosis
Vascular Diseases
Carotid Arteries

ASJC Scopus subject areas

  • Endocrinology
  • Endocrinology, Diabetes and Metabolism

Cite this

Rosiglitazone reduces serum homocysteine levels, smooth muscle proliferation, and intimal hyperplasia in Sprague-Dawley rats fed a high methionine diet. / Murthy, Subramanyam N.; Obregon, Demian F.; Chattergoon, Natasha; Fonseca, Neil A.; Mondal, Debasis; Dunne, Jeffrey B.; Diez, Jose G.; Jeter, James R.; Kadowitz, Philip J.; Agrawal, Krishna C.; McNamara, Dennis B.; Fonseca, Vivian A.

In: Metabolism: Clinical and Experimental, Vol. 54, No. 5, 05.2005, p. 645-652.

Research output: Contribution to journalArticle

Murthy, SN, Obregon, DF, Chattergoon, N, Fonseca, NA, Mondal, D, Dunne, JB, Diez, JG, Jeter, JR, Kadowitz, PJ, Agrawal, KC, McNamara, DB & Fonseca, VA 2005, 'Rosiglitazone reduces serum homocysteine levels, smooth muscle proliferation, and intimal hyperplasia in Sprague-Dawley rats fed a high methionine diet', Metabolism: Clinical and Experimental, vol. 54, no. 5, pp. 645-652. https://doi.org/10.1016/j.metabol.2004.12.008
Murthy, Subramanyam N. ; Obregon, Demian F. ; Chattergoon, Natasha ; Fonseca, Neil A. ; Mondal, Debasis ; Dunne, Jeffrey B. ; Diez, Jose G. ; Jeter, James R. ; Kadowitz, Philip J. ; Agrawal, Krishna C. ; McNamara, Dennis B. ; Fonseca, Vivian A. / Rosiglitazone reduces serum homocysteine levels, smooth muscle proliferation, and intimal hyperplasia in Sprague-Dawley rats fed a high methionine diet. In: Metabolism: Clinical and Experimental. 2005 ; Vol. 54, No. 5. pp. 645-652.
@article{2f80cf5948a64201b87d55eef849307c,
title = "Rosiglitazone reduces serum homocysteine levels, smooth muscle proliferation, and intimal hyperplasia in Sprague-Dawley rats fed a high methionine diet",
abstract = "Homocysteine (Hcy) is a metabolite of the essential amino acid methionine. Hyperhomocysteinemia is associated with vascular disease, particularly carotid stenosis. Rosiglitazone, a ligand of the peroxisome proliferator-activated receptor γ, attenuates balloon catheter-induced carotid intimal hyperplasia in type 2 diabetic rats. We studied 4 groups (n = 7 per group) of adult female Sprague-Dawley rats fed (a) powdered laboratory chow (control), (b) control diet with rosiglitazone (3.0 mg/kg/d), (c) diet containing 1.0{\%} l-methionine, and (d) diet containing methionine and rosiglitazone. After 1 week on high methionine diet, the rats were administered an aqueous preparation of rosiglitazone by oral gavage. One week after initiation of rosiglitazone, balloon catheter injury of the carotid artery was carried out using established methods, and the animals continued on their respective dietary and drug regimens for another 21 days. At the end of the experimental period, blood samples were collected, and carotid arteries and liver were harvested. Serum Hcy increased significantly on methionine diet compared with controls (28.9 ± 3.2 vs 6.3 ± 0.04 μmol/L). Development of intimal hyperplasia was 4-fold higher in methionine-fed rats; this augmentation was significantly reduced (P",
author = "Murthy, {Subramanyam N.} and Obregon, {Demian F.} and Natasha Chattergoon and Fonseca, {Neil A.} and Debasis Mondal and Dunne, {Jeffrey B.} and Diez, {Jose G.} and Jeter, {James R.} and Kadowitz, {Philip J.} and Agrawal, {Krishna C.} and McNamara, {Dennis B.} and Fonseca, {Vivian A.}",
year = "2005",
month = "5",
doi = "10.1016/j.metabol.2004.12.008",
language = "English (US)",
volume = "54",
pages = "645--652",
journal = "Metabolism: Clinical and Experimental",
issn = "0026-0495",
publisher = "W.B. Saunders Ltd",
number = "5",

}

TY - JOUR

T1 - Rosiglitazone reduces serum homocysteine levels, smooth muscle proliferation, and intimal hyperplasia in Sprague-Dawley rats fed a high methionine diet

AU - Murthy, Subramanyam N.

AU - Obregon, Demian F.

AU - Chattergoon, Natasha

AU - Fonseca, Neil A.

AU - Mondal, Debasis

AU - Dunne, Jeffrey B.

AU - Diez, Jose G.

AU - Jeter, James R.

AU - Kadowitz, Philip J.

AU - Agrawal, Krishna C.

AU - McNamara, Dennis B.

AU - Fonseca, Vivian A.

PY - 2005/5

Y1 - 2005/5

N2 - Homocysteine (Hcy) is a metabolite of the essential amino acid methionine. Hyperhomocysteinemia is associated with vascular disease, particularly carotid stenosis. Rosiglitazone, a ligand of the peroxisome proliferator-activated receptor γ, attenuates balloon catheter-induced carotid intimal hyperplasia in type 2 diabetic rats. We studied 4 groups (n = 7 per group) of adult female Sprague-Dawley rats fed (a) powdered laboratory chow (control), (b) control diet with rosiglitazone (3.0 mg/kg/d), (c) diet containing 1.0% l-methionine, and (d) diet containing methionine and rosiglitazone. After 1 week on high methionine diet, the rats were administered an aqueous preparation of rosiglitazone by oral gavage. One week after initiation of rosiglitazone, balloon catheter injury of the carotid artery was carried out using established methods, and the animals continued on their respective dietary and drug regimens for another 21 days. At the end of the experimental period, blood samples were collected, and carotid arteries and liver were harvested. Serum Hcy increased significantly on methionine diet compared with controls (28.9 ± 3.2 vs 6.3 ± 0.04 μmol/L). Development of intimal hyperplasia was 4-fold higher in methionine-fed rats; this augmentation was significantly reduced (P

AB - Homocysteine (Hcy) is a metabolite of the essential amino acid methionine. Hyperhomocysteinemia is associated with vascular disease, particularly carotid stenosis. Rosiglitazone, a ligand of the peroxisome proliferator-activated receptor γ, attenuates balloon catheter-induced carotid intimal hyperplasia in type 2 diabetic rats. We studied 4 groups (n = 7 per group) of adult female Sprague-Dawley rats fed (a) powdered laboratory chow (control), (b) control diet with rosiglitazone (3.0 mg/kg/d), (c) diet containing 1.0% l-methionine, and (d) diet containing methionine and rosiglitazone. After 1 week on high methionine diet, the rats were administered an aqueous preparation of rosiglitazone by oral gavage. One week after initiation of rosiglitazone, balloon catheter injury of the carotid artery was carried out using established methods, and the animals continued on their respective dietary and drug regimens for another 21 days. At the end of the experimental period, blood samples were collected, and carotid arteries and liver were harvested. Serum Hcy increased significantly on methionine diet compared with controls (28.9 ± 3.2 vs 6.3 ± 0.04 μmol/L). Development of intimal hyperplasia was 4-fold higher in methionine-fed rats; this augmentation was significantly reduced (P

UR - http://www.scopus.com/inward/record.url?scp=20944432916&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=20944432916&partnerID=8YFLogxK

U2 - 10.1016/j.metabol.2004.12.008

DO - 10.1016/j.metabol.2004.12.008

M3 - Article

C2 - 15877295

AN - SCOPUS:20944432916

VL - 54

SP - 645

EP - 652

JO - Metabolism: Clinical and Experimental

JF - Metabolism: Clinical and Experimental

SN - 0026-0495

IS - 5

ER -