Roles of helper and defective retroviral genomes in murine erythroleukemia: Studies of spleen focus-forming virus in the absence of helper

R. K. Bestwick, W. D. Hankins, David Kabat

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

Retroviruses that cause acute oncogenesis are generally complexes of a replication-competent helper virus and a replication-defective component. However, the pure defective components have not been previously available. We prepared the defective spleen focus-forming virus component of Rauscher erythroleukemia virus (R-SFFV) by transfecting a colinear R-SFFV DNA clone into a retroviral packaging cell line (ψ2 cells). The transfected cells released virus (ψ2/SFFV) that was free of helper virus and that induced erythropoietin-dependent erythroid burst formation in bone marrow cultures. When injected into normal adult NIH/Swiss mice in moderate doses, ψ2/SFFV caused a rapid splenic erythroblastosis that regressed. Extensive erythroblastosis could be maintained by repeated injections of ψ2/SFFV into anemic mice or by the addition of a helper virus. We conclude that R-SFFV alone causes proliferation but not immortalization of a population of erythroblasts that is normally replenished from a precursor stem cell pool. Because these precursor cells are inefficiently infected, a single moderate inoculum of ψ2/SFFV causes a wave of erythroblastosis. The properties of the proliferating erythroblasts are substantially determined by the R-SFFV viral component.

Original languageEnglish (US)
Pages (from-to)660-664
Number of pages5
JournalJournal of Virology
Volume56
Issue number3
StatePublished - 1985

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Spleen Focus-Forming Viruses
Leukemia, Erythroblastic, Acute
spleen
Genome
viruses
genome
mice
erythroblasts
Helper Viruses
Viral Structures
Erythroblasts
Retroviridae
erythropoietin
cells
Rauscher Virus
bone marrow
carcinogenesis
packaging
Defective Viruses
stem cells

ASJC Scopus subject areas

  • Immunology

Cite this

Roles of helper and defective retroviral genomes in murine erythroleukemia : Studies of spleen focus-forming virus in the absence of helper. / Bestwick, R. K.; Hankins, W. D.; Kabat, David.

In: Journal of Virology, Vol. 56, No. 3, 1985, p. 660-664.

Research output: Contribution to journalArticle

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