Roles of Arabidopsis patatin-related phospholipases a in root development are related to auxin responses and phosphate deficiency

Steffen Rietz, Georgi Dermendjiev, Esther Oppermann, Fikadu Tafesse, Yunus Effendi, André Holk, Jane E. Parker, Markus Teige, G. F E Scherer

Research output: Contribution to journalArticle

57 Citations (Scopus)

Abstract

Phospholipase A enzymes cleave phospho- and galactolipids to generate free fatty acids and lysolipids that function in animal and plant hormone signaling. Here, we describe three Arabidopsis patatin-related phospholipase A (pPLA) genes AtPLAIVA, AtPLAIVB, and AtPLAIVC and their corresponding proteins. Loss-of-function mutants reveal roles for these pPLAs in roots during normal development and under phosphate deprivation. AtPLAIVA is expressed strongly and exclusively in roots and AtplaIVA-null mutants have reduced lateral root development, characteristic of an impaired auxin response. By contrast, AtPLAIVB is expressed weakly in roots, cotyledons, and leaves but is transcriptionally induced by auxin, although AtplaIVB mutants develop normally. AtPLAIVC is expressed in the floral gynaecium and is induced by abscisic acid (ABA) or phosphate deficiency in roots. While an AtplaIVC-1 loss-of-function mutant displays ABA responsiveness, it exhibits an impaired response to phosphate deficiency during root development. Recombinant AtPLA proteins hydrolyze preferentially galactolipids and, less efficiently, phospholipids, although these enzymes are not localized in chloroplasts. We find that AtPLAIVA and AtPLAIVB are phosphorylated by calcium-dependent protein kinases in vitro and this enhances their activities on phosphatidylcholine but not on phosphatidylglycerol. Taken together, the data reveal novel functions of pPLAs in root development with individual roles at the interface between phosphate deficiency and auxin signaling.

Original languageEnglish (US)
Pages (from-to)524-538
Number of pages15
JournalMolecular Plant
Volume3
Issue number3
DOIs
StatePublished - May 2010
Externally publishedYes

Fingerprint

patatin
Indoleacetic Acids
Phospholipases
Arabidopsis
auxins
Galactolipids
Phosphates
phosphates
Abscisic Acid
galactolipids
vif Genes
mutants
Phosphatidylglycerols
Plant Growth Regulators
Cotyledon
Phospholipases A
Enzymes
Chloroplasts
Phosphatidylcholines
abscisic acid

Keywords

  • Abiotic/environmental stress
  • Arabidopsis
  • Gene regulation
  • Hormonal regulation
  • Protein phosphorylation/dephosphorylation
  • Signal transduction

ASJC Scopus subject areas

  • Plant Science
  • Molecular Biology

Cite this

Roles of Arabidopsis patatin-related phospholipases a in root development are related to auxin responses and phosphate deficiency. / Rietz, Steffen; Dermendjiev, Georgi; Oppermann, Esther; Tafesse, Fikadu; Effendi, Yunus; Holk, André; Parker, Jane E.; Teige, Markus; Scherer, G. F E.

In: Molecular Plant, Vol. 3, No. 3, 05.2010, p. 524-538.

Research output: Contribution to journalArticle

Rietz, S, Dermendjiev, G, Oppermann, E, Tafesse, F, Effendi, Y, Holk, A, Parker, JE, Teige, M & Scherer, GFE 2010, 'Roles of Arabidopsis patatin-related phospholipases a in root development are related to auxin responses and phosphate deficiency', Molecular Plant, vol. 3, no. 3, pp. 524-538. https://doi.org/10.1093/mp/ssp109
Rietz, Steffen ; Dermendjiev, Georgi ; Oppermann, Esther ; Tafesse, Fikadu ; Effendi, Yunus ; Holk, André ; Parker, Jane E. ; Teige, Markus ; Scherer, G. F E. / Roles of Arabidopsis patatin-related phospholipases a in root development are related to auxin responses and phosphate deficiency. In: Molecular Plant. 2010 ; Vol. 3, No. 3. pp. 524-538.
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