Role of the retinal vascular endothelial cell in ocular disease

Arpita S. Bharadwaj, Binoy Appukuttan, Phillip Wilmarth, Yuzhen Pan, Andrew J. Stempel, Timothy J. Chipps, Eric E. Benedetti, David O. Zamora, Dongseok Choi, Larry David, Justine R. Smith

Research output: Contribution to journalArticle

59 Citations (Scopus)

Abstract

Retinal endothelial cells line the arborizing microvasculature that supplies and drains the neural retina. The anatomical and physiological characteristics of these endothelial cells are consistent with nutritional requirements and protection of a tissue critical to vision. On the one hand, the endothelium must ensure the supply of oxygen and other nutrients to the metabolically active retina, and allow access to circulating cells that maintain the vasculature or survey the retina for the presence of potential pathogens. On the other hand, the endothelium contributes to the blood-retinal barrier that protects the retina by excluding circulating molecular toxins, microorganisms, and pro-inflammatory leukocytes. Features required to fulfill these functions may also predispose to disease processes, such as retinal vascular leakage and neovascularization, and trafficking of microbes and inflammatory cells. Thus, the retinal endothelial cell is a key participant in retinal ischemic vasculopathies that include diabetic retinopathy and retinopathy of prematurity, and retinal inflammation or infection, as occurs in posterior uveitis. Using gene expression and proteomic profiling, it has been possible to explore the molecular phenotype of the human retinal endothelial cell and contribute to understanding of the pathogenesis of these diseases. In addition to providing support for the involvement of well-characterized endothelial molecules, profiling has the power to identify new players in retinal pathologies. Findings may have implications for the design of new biological therapies. Additional progress in this field is anticipated as other technologies, including epigenetic profiling methods, whole transcriptome shotgun sequencing, and metabolomics, are used to study the human retinal endothelial cell.

Original languageEnglish (US)
Pages (from-to)102-180
Number of pages79
JournalProgress in Retinal and Eye Research
Volume32
Issue number1
DOIs
StatePublished - Jan 2013

Fingerprint

Retinal Vessels
Eye Diseases
Endothelial Cells
Retina
Endothelium
Posterior Uveitis
Blood-Retinal Barrier
Retinal Neovascularization
Nutritional Requirements
Retinopathy of Prematurity
Biological Therapy
Metabolomics
Firearms
Gene Expression Profiling
Diabetic Retinopathy
Microvessels
Transcriptome
Epigenomics
Proteomics
Leukocytes

Keywords

  • Autoimmune uveitis
  • Diabetic retinopathy
  • Endothelial cell
  • Infectious uveitis
  • Molecular profiling
  • Posterior uveitis
  • Retina
  • Retinopathy of prematurity

ASJC Scopus subject areas

  • Sensory Systems
  • Ophthalmology

Cite this

Bharadwaj, A. S., Appukuttan, B., Wilmarth, P., Pan, Y., Stempel, A. J., Chipps, T. J., ... Smith, J. R. (2013). Role of the retinal vascular endothelial cell in ocular disease. Progress in Retinal and Eye Research, 32(1), 102-180. https://doi.org/10.1016/j.preteyeres.2012.08.004

Role of the retinal vascular endothelial cell in ocular disease. / Bharadwaj, Arpita S.; Appukuttan, Binoy; Wilmarth, Phillip; Pan, Yuzhen; Stempel, Andrew J.; Chipps, Timothy J.; Benedetti, Eric E.; Zamora, David O.; Choi, Dongseok; David, Larry; Smith, Justine R.

In: Progress in Retinal and Eye Research, Vol. 32, No. 1, 01.2013, p. 102-180.

Research output: Contribution to journalArticle

Bharadwaj, AS, Appukuttan, B, Wilmarth, P, Pan, Y, Stempel, AJ, Chipps, TJ, Benedetti, EE, Zamora, DO, Choi, D, David, L & Smith, JR 2013, 'Role of the retinal vascular endothelial cell in ocular disease', Progress in Retinal and Eye Research, vol. 32, no. 1, pp. 102-180. https://doi.org/10.1016/j.preteyeres.2012.08.004
Bharadwaj, Arpita S. ; Appukuttan, Binoy ; Wilmarth, Phillip ; Pan, Yuzhen ; Stempel, Andrew J. ; Chipps, Timothy J. ; Benedetti, Eric E. ; Zamora, David O. ; Choi, Dongseok ; David, Larry ; Smith, Justine R. / Role of the retinal vascular endothelial cell in ocular disease. In: Progress in Retinal and Eye Research. 2013 ; Vol. 32, No. 1. pp. 102-180.
@article{d3fdf3e7b67c453d9e992c4b61483ba8,
title = "Role of the retinal vascular endothelial cell in ocular disease",
abstract = "Retinal endothelial cells line the arborizing microvasculature that supplies and drains the neural retina. The anatomical and physiological characteristics of these endothelial cells are consistent with nutritional requirements and protection of a tissue critical to vision. On the one hand, the endothelium must ensure the supply of oxygen and other nutrients to the metabolically active retina, and allow access to circulating cells that maintain the vasculature or survey the retina for the presence of potential pathogens. On the other hand, the endothelium contributes to the blood-retinal barrier that protects the retina by excluding circulating molecular toxins, microorganisms, and pro-inflammatory leukocytes. Features required to fulfill these functions may also predispose to disease processes, such as retinal vascular leakage and neovascularization, and trafficking of microbes and inflammatory cells. Thus, the retinal endothelial cell is a key participant in retinal ischemic vasculopathies that include diabetic retinopathy and retinopathy of prematurity, and retinal inflammation or infection, as occurs in posterior uveitis. Using gene expression and proteomic profiling, it has been possible to explore the molecular phenotype of the human retinal endothelial cell and contribute to understanding of the pathogenesis of these diseases. In addition to providing support for the involvement of well-characterized endothelial molecules, profiling has the power to identify new players in retinal pathologies. Findings may have implications for the design of new biological therapies. Additional progress in this field is anticipated as other technologies, including epigenetic profiling methods, whole transcriptome shotgun sequencing, and metabolomics, are used to study the human retinal endothelial cell.",
keywords = "Autoimmune uveitis, Diabetic retinopathy, Endothelial cell, Infectious uveitis, Molecular profiling, Posterior uveitis, Retina, Retinopathy of prematurity",
author = "Bharadwaj, {Arpita S.} and Binoy Appukuttan and Phillip Wilmarth and Yuzhen Pan and Stempel, {Andrew J.} and Chipps, {Timothy J.} and Benedetti, {Eric E.} and Zamora, {David O.} and Dongseok Choi and Larry David and Smith, {Justine R.}",
year = "2013",
month = "1",
doi = "10.1016/j.preteyeres.2012.08.004",
language = "English (US)",
volume = "32",
pages = "102--180",
journal = "Progress in Retinal and Eye Research",
issn = "1350-9462",
publisher = "Elsevier Limited",
number = "1",

}

TY - JOUR

T1 - Role of the retinal vascular endothelial cell in ocular disease

AU - Bharadwaj, Arpita S.

AU - Appukuttan, Binoy

AU - Wilmarth, Phillip

AU - Pan, Yuzhen

AU - Stempel, Andrew J.

AU - Chipps, Timothy J.

AU - Benedetti, Eric E.

AU - Zamora, David O.

AU - Choi, Dongseok

AU - David, Larry

AU - Smith, Justine R.

PY - 2013/1

Y1 - 2013/1

N2 - Retinal endothelial cells line the arborizing microvasculature that supplies and drains the neural retina. The anatomical and physiological characteristics of these endothelial cells are consistent with nutritional requirements and protection of a tissue critical to vision. On the one hand, the endothelium must ensure the supply of oxygen and other nutrients to the metabolically active retina, and allow access to circulating cells that maintain the vasculature or survey the retina for the presence of potential pathogens. On the other hand, the endothelium contributes to the blood-retinal barrier that protects the retina by excluding circulating molecular toxins, microorganisms, and pro-inflammatory leukocytes. Features required to fulfill these functions may also predispose to disease processes, such as retinal vascular leakage and neovascularization, and trafficking of microbes and inflammatory cells. Thus, the retinal endothelial cell is a key participant in retinal ischemic vasculopathies that include diabetic retinopathy and retinopathy of prematurity, and retinal inflammation or infection, as occurs in posterior uveitis. Using gene expression and proteomic profiling, it has been possible to explore the molecular phenotype of the human retinal endothelial cell and contribute to understanding of the pathogenesis of these diseases. In addition to providing support for the involvement of well-characterized endothelial molecules, profiling has the power to identify new players in retinal pathologies. Findings may have implications for the design of new biological therapies. Additional progress in this field is anticipated as other technologies, including epigenetic profiling methods, whole transcriptome shotgun sequencing, and metabolomics, are used to study the human retinal endothelial cell.

AB - Retinal endothelial cells line the arborizing microvasculature that supplies and drains the neural retina. The anatomical and physiological characteristics of these endothelial cells are consistent with nutritional requirements and protection of a tissue critical to vision. On the one hand, the endothelium must ensure the supply of oxygen and other nutrients to the metabolically active retina, and allow access to circulating cells that maintain the vasculature or survey the retina for the presence of potential pathogens. On the other hand, the endothelium contributes to the blood-retinal barrier that protects the retina by excluding circulating molecular toxins, microorganisms, and pro-inflammatory leukocytes. Features required to fulfill these functions may also predispose to disease processes, such as retinal vascular leakage and neovascularization, and trafficking of microbes and inflammatory cells. Thus, the retinal endothelial cell is a key participant in retinal ischemic vasculopathies that include diabetic retinopathy and retinopathy of prematurity, and retinal inflammation or infection, as occurs in posterior uveitis. Using gene expression and proteomic profiling, it has been possible to explore the molecular phenotype of the human retinal endothelial cell and contribute to understanding of the pathogenesis of these diseases. In addition to providing support for the involvement of well-characterized endothelial molecules, profiling has the power to identify new players in retinal pathologies. Findings may have implications for the design of new biological therapies. Additional progress in this field is anticipated as other technologies, including epigenetic profiling methods, whole transcriptome shotgun sequencing, and metabolomics, are used to study the human retinal endothelial cell.

KW - Autoimmune uveitis

KW - Diabetic retinopathy

KW - Endothelial cell

KW - Infectious uveitis

KW - Molecular profiling

KW - Posterior uveitis

KW - Retina

KW - Retinopathy of prematurity

UR - http://www.scopus.com/inward/record.url?scp=84871394516&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84871394516&partnerID=8YFLogxK

U2 - 10.1016/j.preteyeres.2012.08.004

DO - 10.1016/j.preteyeres.2012.08.004

M3 - Article

C2 - 22982179

AN - SCOPUS:84871394516

VL - 32

SP - 102

EP - 180

JO - Progress in Retinal and Eye Research

JF - Progress in Retinal and Eye Research

SN - 1350-9462

IS - 1

ER -