Role of the pJM1 plasmid-encoded transport proteins FatB, C and D in ferric anguibactin uptake in the fish pathogen Vibrio anguillarum

Hiroaki Naka, Claudia Lopez, Jorge H. Crosa

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

Vibrio anguillarum serotype O1 is part of the natural flora in the aquatic habitat, but under certain circumstances it can cause terminal haemorrhagic septicemia in marine and fresh water fish due to the action of the anguibactin iron uptake system encoded by the virulence plasmid pJM1. This plasmid harbours the genes for the biosynthesis of the siderophore anguibactin and the ferric anguibactin transport proteins FatD, C, B and A encoded in the iron transport operon. The FatA protein is the outer membrane receptor for the ferric siderophore complex and the FatB lipoprotein provides the periplasmic domain for its internalization, whereas the FatC and D proteins are located in the cytoplasmic membrane and might play a role as part of the ABC transporter for internalization of the ferric siderophore. In this work we demonstrate the essential role of these two inner membrane proteins in ferric anguibactin transport and that the lipoprotein nature of FatB is not necessary for ferric anguibactin transport.

Original languageEnglish (US)
Pages (from-to)104-111
Number of pages8
JournalEnvironmental Microbiology Reports
Volume2
Issue number1
DOIs
StatePublished - Feb 2010

Fingerprint

Vibrio anguillarum
Vibrio
siderophores
transport proteins
Protein C
plasmid
siderophore
plasmids
Carrier Proteins
Fishes
Plasmids
pathogen
uptake mechanisms
lipoproteins
protein
pathogens
fish
membrane
Siderophores
iron

ASJC Scopus subject areas

  • Agricultural and Biological Sciences (miscellaneous)
  • Ecology, Evolution, Behavior and Systematics

Cite this

Role of the pJM1 plasmid-encoded transport proteins FatB, C and D in ferric anguibactin uptake in the fish pathogen Vibrio anguillarum. / Naka, Hiroaki; Lopez, Claudia; Crosa, Jorge H.

In: Environmental Microbiology Reports, Vol. 2, No. 1, 02.2010, p. 104-111.

Research output: Contribution to journalArticle

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