Role of the bZIP Transcription Factor IREBF1 in the NGF Induction of Stromelysin-1 (Transin) Gene Expression in PC12 Cells

Sunita DeSouza, Lora A. Nordstrom, Gary Ciment

Research output: Contribution to journalArticlepeer-review

3 Scopus citations

Abstract

Stromelysin-1 (ST-1) is one of the most nerve growth factor- (NGF) responsive gene products expressed in PC12 cells. In previous work, we identified a novel NGF-responsive element in the proximal promoter region of the ST-1 gene that participates in this induction, and showed that it bound a protein present in the nuclei of PC12 cells. Here, we identify a transcription factor that specifically recognizes this regulatory element - the interferon-response element binding factor-1 (IREBF1), a member of the basic leucine zipper gene family. We show that IREBF1 is constitutively expressed in PC12 cells and that overexpression of IREBF1 augments NGF-responsive ST-1 gene regulation, but does not affect basal levels of expression. On the other hand, expression of a mutated form of this transcription factor lacking the DNA binding domain attenuated NGF responsiveness, without affecting basal levels of expression. These data suggest that IREBF1 is part of the NGF-responsive transcriptional machinery necessary for the expression of ST-1 in PC12 cells.

Original languageEnglish (US)
Pages (from-to)243-255
Number of pages13
JournalJournal of Molecular Neuroscience
Volume8
Issue number3
DOIs
StatePublished - Jan 1 1997

Keywords

  • Gene regulation
  • Interferon response element binding factor
  • Leucine zipper
  • Matrix metalloproteinase
  • Nerve growth factor
  • PC12 cells
  • Stromelysin
  • Transcription factor

ASJC Scopus subject areas

  • Cellular and Molecular Neuroscience

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