Role of Salmonella typhimurium mn-superoxide dismutase (SodA) in protection against early killing by J774 macrophages

R. M. Tsolis, A. J. Baumler, F. Heffron

    Research output: Contribution to journalArticle

    83 Scopus citations

    Abstract

    The Salmonella typhimurium gene for Mn-cofactored superoxide dismutase (sodA) was cloned by complementation of an Escherichia coli soda sodB mutant for growth on minimal medium. Sequence analysis revealed an open reading frame of 618 bp encoding a polypeptide with 97% identity to E. coli SodA. A S. typhimurium soda mutant was created by allelic exchange and tested for the ability to survive in the murine macrophage-like cell line J774. Growth of bacteria under iron-limiting conditions, inactivation of the Fur repressor, or expression of sodA from a plasmid resulted in increased resistance to early killing by J774 cells, which was abolished in the soda mutant. These results suggest that resistance to the early oxygen-dependent microbicidal mechanisms of phagocytes involves the SodA gene product. The S. typhimurium sodA mutant was not significantly attenuated in mice, however, which suggests that resistance to early oxygen-dependent microbicidal mechanisms in vivo may play only a minor role in Salmonella pathogenesis.

    Original languageEnglish (US)
    Pages (from-to)1739-1744
    Number of pages6
    JournalInfection and Immunity
    Volume63
    Issue number5
    DOIs
    StatePublished - 1995

    ASJC Scopus subject areas

    • Parasitology
    • Microbiology
    • Immunology
    • Infectious Diseases

    Fingerprint Dive into the research topics of 'Role of Salmonella typhimurium mn-superoxide dismutase (SodA) in protection against early killing by J774 macrophages'. Together they form a unique fingerprint.

  • Cite this