Role of moving growth cone-like 'wave' structures in the outgrowth of cultured hippocampal axons and dendrites

Gordon Ruthel, Gary Banker

Research output: Contribution to journalArticle

70 Scopus citations

Abstract

Hippocampal neurons exhibit periodically recurring growth cone-like structures, referred to as 'waves,' that emerge at the base of neurites and travel distally to the tip. As a wave nears the tip, the neurite undergoes retraction, and when it reaches the tip, the neurite undergoes a burst of growth. At 1 day in culture, during early axon outgrowth, axons undergo an average 7.5-μm retraction immediately preceding wave arrival at the tip followed by 12-μm growth immediately after arrival (an average net growth of 4.5 μm). In branched axons, waves often selectively travel down one branch or the other. Growth selectively occurs in the branch chosen by the wave. In dendrites, which grow much slower on average, wave-associated retractions are much greater, resulting in less net growth. In the presence of Brefeldin A, which disrupts membrane traffic through the Golgi apparatus and leads to retraction of the axon, axonal waves continue to be associated with both growth spurts and retractions. The magnitude of the growth spurts is not significantly different from untreated axons, but wave-associated retractions are significantly increased. The close association between waves and cyclical elongation suggests that waves may act to bring about this pattern of growth. Our results also show that modulation of regularly occurring retraction phases plays a prominent role in determining average outgrowth rates.

Original languageEnglish (US)
Pages (from-to)97-106
Number of pages10
JournalJournal of Neurobiology
Volume39
Issue number1
DOIs
StatePublished - Apr 13 1999

Keywords

  • Brefeldin A
  • Dendrite
  • Growth cone
  • Neurite outgrowth

ASJC Scopus subject areas

  • Neuroscience(all)
  • Cellular and Molecular Neuroscience

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