Role of membrane immunoglobulin (Ig) crosslinking in membrane Ig-mediated, major histocompatibility-restricted T cell-B cell cooperation

H. P. Tony, N. E. Phillips, David Parker

Research output: Contribution to journalArticle

114 Citations (Scopus)

Abstract

Resting murine B lymphocytes can present rabbit anti-Ig to T cell lines specific for normal rabbit globulin. The T cell-B cell interaction is major histocompatibility complex (MHC)-restricted, and leads to activation, proliferation, and differentiation of the resting B cell into an antibody-secreting cell. Efficient antigen presentation and B cell activation depends upon binding of rabbit globulin to (membrane) mIg. To investigate the role of mIg in this polyclonal model for a T cell-dependent primary antibody response, we determined whether crosslinking of mIg is required either for efficient antigen presentation, as measured by helper T cell activation, or for the B cell response to T cell help, since all the direct effects of anti-Ig on B cells require crosslinking of mIg. We found that monovalent Fab' fragments of anti-IgM or anti-IgD work as efficiently as their divalent counterparts. Therefore, a signal transduced through the antigen receptor seems not be required when T cell help is provided by an MHC-restricted T helper cell recognizing antigen on the B cell surface. Moreover, rabbit globulin bound to class I MHC molecules in the form of anti-H-2K also results in efficient antigen presentation and T cell-dependent B cell activation. However, mIg still appears to be specialized for antigen presentation, since anti-Ig is presented about three- to fivefold more efficiently than anti-H-2K.

Original languageEnglish (US)
Pages (from-to)1695-1708
Number of pages14
JournalJournal of Experimental Medicine
Volume162
Issue number5
StatePublished - 1985
Externally publishedYes

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Histocompatibility
Immunoglobulins
B-Lymphocytes
T-Lymphocytes
Membranes
Antigen Presentation
Globulins
Major Histocompatibility Complex
Rabbits
Helper-Inducer T-Lymphocytes
Antibody-Producing Cells
Immunoglobulin Fab Fragments
Antigen Receptors
Cell Communication
Antibody Formation
Antigens
Cell Line

ASJC Scopus subject areas

  • Immunology

Cite this

Role of membrane immunoglobulin (Ig) crosslinking in membrane Ig-mediated, major histocompatibility-restricted T cell-B cell cooperation. / Tony, H. P.; Phillips, N. E.; Parker, David.

In: Journal of Experimental Medicine, Vol. 162, No. 5, 1985, p. 1695-1708.

Research output: Contribution to journalArticle

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