Role of LytF and AtlS in eDNA Release by Streptococcus gordonii

Yifan Xu, Jens Kreth

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Extracellular DNA (eDNA) is an important component of the biofilm matrix produced by many bacteria. In general, the release of eDNA is associated with the activity of muralytic enzymes leading to obvious cell lysis. In the Gram-positive oral commensal Streptococcus gordonii, eDNA release is dependent on pyruvate oxidase generated hydrogen peroxide (H2O2). Addition of H2O2 to cells grown under conditions non-permissive for H2O2 production causes eDNA release. Furthermore, eDNA release is maximal under aerobic growth conditions known to induce pyruvate oxidase gene expression and H2O2 production. Obvious cell lysis, however, does not occur. Two enzymes have been recently associated with eDNA release in S. gordonii. The autolysin AtlS and the competence regulated murein hydrolase LytF. In the present report, we investigated the role of both proteins in the H2O2 dependent eDNA release process. Single and double mutants in the respective genes for LytF and AtlS released less eDNA under normal growth conditions, but the AtlS mutant was still inducible for eDNA release by external H2O2. Moreover, we showed that the AtlS mutation interfered with the ability of S. gordonii to produce eDNA release inducing amounts of H2O2. Our data support a role of LytF in the H2O2 eDNA dependent release of S. gordonii as part of the competence stress pathway responding to oxidative stress.

Original languageEnglish (US)
Article numbere62339
JournalPLoS One
Volume8
Issue number4
DOIs
StatePublished - Apr 24 2013
Externally publishedYes

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Streptococcus gordonii
DNA
Pyruvate Oxidase
N-Acetylmuramoyl-L-alanine Amidase
Mental Competency
mutants
Aptitude
Oxidative stress
aerobiosis
peptidoglycans
Biofilms
cells
Enzymes
Growth
hydrolases
Gene expression

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

Cite this

Role of LytF and AtlS in eDNA Release by Streptococcus gordonii. / Xu, Yifan; Kreth, Jens.

In: PLoS One, Vol. 8, No. 4, e62339, 24.04.2013.

Research output: Contribution to journalArticle

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