Role of luminal nutrients and endogenous GLP-2 in intestinal adaptation to mid-small bowel resection

Elizabeth M. Dahly, Melanie Gillingham, Ziwen Guo, Sangita G. Murali, David W. Nelson, Jens J. Holst, Denise M. Ney

Research output: Contribution to journalArticle

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Abstract

To elucidate the role of luminal nutrients and glucagon-like peptide-2 (GLP-2) in intestinal adaptation, rats were subjected to 70% midjejunoileal resection or ileal transection and were maintained with total parenteral nutrition (TPN) or oral feeding. TPN rats showed small bowel mucosal hyperplasia at 8 h through 7 days after resection, demonstrating that exogenous luminal nutrients are not essential for resection-induced adaptation when residual ileum and colon are present. Increased enterocyte proliferation was a stronger determinant of resection-induced mucosal growth in orally fed animals, whereas decreased apoptosis showed a greater effect in TPN animals. Resection induced significant transient increases in plasma bioactive GLP-2 during TPN, whereas resection induced sustained increases in plasma GLP-2 during oral feeding. Resection-induced adaptive growth in TPN and orally fed rats was associated with a significant positive correlation between increases in plasma bioactive GLP-2 and proglucagon mRNA expression in the colon of TPN rats and ileum of orally fed rats. These data support a significant role for endogenous GLP-2 in the adaptive response to mid-small bowel resection in both TPN and orally fed rats.

Original languageEnglish (US)
JournalAmerican Journal of Physiology - Gastrointestinal and Liver Physiology
Volume284
Issue number4 47-4
StatePublished - Apr 1 2003
Externally publishedYes

Fingerprint

Glucagon-Like Peptide 2
Total Parenteral Nutrition
Food
Ileum
Colon
Proglucagon
Enterocytes
Growth
Hyperplasia
Apoptosis
Messenger RNA

Keywords

  • Enterocyte proliferation and apoptosis
  • Glucagon-like peptide-2
  • Ileum
  • Parenteral nutrition
  • Proglucagon

ASJC Scopus subject areas

  • Gastroenterology
  • Physiology

Cite this

Role of luminal nutrients and endogenous GLP-2 in intestinal adaptation to mid-small bowel resection. / Dahly, Elizabeth M.; Gillingham, Melanie; Guo, Ziwen; Murali, Sangita G.; Nelson, David W.; Holst, Jens J.; Ney, Denise M.

In: American Journal of Physiology - Gastrointestinal and Liver Physiology, Vol. 284, No. 4 47-4, 01.04.2003.

Research output: Contribution to journalArticle

Dahly, Elizabeth M. ; Gillingham, Melanie ; Guo, Ziwen ; Murali, Sangita G. ; Nelson, David W. ; Holst, Jens J. ; Ney, Denise M. / Role of luminal nutrients and endogenous GLP-2 in intestinal adaptation to mid-small bowel resection. In: American Journal of Physiology - Gastrointestinal and Liver Physiology. 2003 ; Vol. 284, No. 4 47-4.
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