Role of isosafrole as complexing agent and inducer of P-450LM4 in rabbit liver microsomes

Marcel Delaforge, Dennis R. Koop, Minor J. Coon

Research output: Contribution to journalArticle

11 Scopus citations

Abstract

Isosafrole serves as an excellent inducing agent in rabbits for P-450LM4, the same isozyme as that which is induced by 3-methylcholanthrene and 5,6-benzoflavone. Since the isosafrole adduct formed with isozyme 4 is unstable, the uncomplexed cytochrome may be purified in very good yield (over 30%, based on total P-450) from liver microsomes of animals given this agent. Isosafrole forms adducts with both isozyme 2 (the phenobarbital-inducible form of P-450) and isozyme 4 in the reconstituted system containing NADPH-cytochrome P-450 reductase, phosphatidylcholine, and NADPH under aerobic conditions, but with the latter cytochrome the reaction has a lower Km and maximal rate.

Original languageEnglish (US)
Pages (from-to)59-65
Number of pages7
JournalBiochemical and Biophysical Research Communications
Volume108
Issue number1
DOIs
StatePublished - Sep 16 1982

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ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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