TY - JOUR
T1 - Role of isosafrole as complexing agent and inducer of P-450LM4 in rabbit liver microsomes
AU - Delaforge, Marcel
AU - Koop, Dennis R.
AU - Coon, Minor J.
N1 - Funding Information:
This research was supported by Grant tutes of Health. We are grateful to Sharon purifying NADPH-cytochrome P-450 reductase ing P-450~~2 and P-450~4 from rabbit liver Foundation for travel funds provided to M.
PY - 1982/9/16
Y1 - 1982/9/16
N2 - Isosafrole serves as an excellent inducing agent in rabbits for P-450LM4, the same isozyme as that which is induced by 3-methylcholanthrene and 5,6-benzoflavone. Since the isosafrole adduct formed with isozyme 4 is unstable, the uncomplexed cytochrome may be purified in very good yield (over 30%, based on total P-450) from liver microsomes of animals given this agent. Isosafrole forms adducts with both isozyme 2 (the phenobarbital-inducible form of P-450) and isozyme 4 in the reconstituted system containing NADPH-cytochrome P-450 reductase, phosphatidylcholine, and NADPH under aerobic conditions, but with the latter cytochrome the reaction has a lower Km and maximal rate.
AB - Isosafrole serves as an excellent inducing agent in rabbits for P-450LM4, the same isozyme as that which is induced by 3-methylcholanthrene and 5,6-benzoflavone. Since the isosafrole adduct formed with isozyme 4 is unstable, the uncomplexed cytochrome may be purified in very good yield (over 30%, based on total P-450) from liver microsomes of animals given this agent. Isosafrole forms adducts with both isozyme 2 (the phenobarbital-inducible form of P-450) and isozyme 4 in the reconstituted system containing NADPH-cytochrome P-450 reductase, phosphatidylcholine, and NADPH under aerobic conditions, but with the latter cytochrome the reaction has a lower Km and maximal rate.
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U2 - 10.1016/0006-291X(82)91831-9
DO - 10.1016/0006-291X(82)91831-9
M3 - Article
C2 - 6816232
AN - SCOPUS:0020363996
SN - 0006-291X
VL - 108
SP - 59
EP - 65
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
IS - 1
ER -