Role of GH and IGF-I in the regulation of IGF-I, IGF-I receptor and IGF binding protein gene expression in the rat spleen

Horacio M. Domené, Rina Meidan, Shoshana Yakar, Zila Shen-Orr, Fernando Cassorla, Charles Roberts, Derek LeRoith

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

To characterize the expression of the IGF-I system in the spleen and its role in spleen growth, we have studied the effect of hypophysectomy and the action of either GH or IGF-I treatment on the expression of several components of the IGF system in the rat. Female Sprague-Dawley rats were hypophysectomized (Hx) on postnatal day 50, and five animals each received twice-daily sc injections of saline, bovine GH (bGH; 84 μg/animal/day), or recombinant human IGF-I (rhIGF-I; 125 μg/animal/day) for 11 days. Compared to sham-operated controls, Hx animals exhibited a reduction in both body (192.6 ± 5.6 g (mean ± S.E.M.) vs. 268.6 ± 6.0 g; P

Original languageEnglish (US)
Pages (from-to)215-226
Number of pages12
JournalRegulatory Peptides
Volume52
Issue number3
DOIs
StatePublished - Aug 4 1994
Externally publishedYes

Fingerprint

Insulin-Like Growth Factor Binding Proteins
IGF Type 1 Receptor
Insulin-Like Growth Factor I
Gene expression
Rats
Animals
Spleen
Gene Expression
Hypophysectomy
Sprague Dawley Rats
Scanning electron microscopy
Injections
Growth
Therapeutics

Keywords

  • Gene expression
  • Insulin-like growth factor
  • Insulin-like growth factor binding protein
  • mRNA level
  • Splenic growth

ASJC Scopus subject areas

  • Biochemistry
  • Endocrinology
  • Physiology
  • Neuroscience(all)

Cite this

Role of GH and IGF-I in the regulation of IGF-I, IGF-I receptor and IGF binding protein gene expression in the rat spleen. / Domené, Horacio M.; Meidan, Rina; Yakar, Shoshana; Shen-Orr, Zila; Cassorla, Fernando; Roberts, Charles; LeRoith, Derek.

In: Regulatory Peptides, Vol. 52, No. 3, 04.08.1994, p. 215-226.

Research output: Contribution to journalArticle

Domené, Horacio M. ; Meidan, Rina ; Yakar, Shoshana ; Shen-Orr, Zila ; Cassorla, Fernando ; Roberts, Charles ; LeRoith, Derek. / Role of GH and IGF-I in the regulation of IGF-I, IGF-I receptor and IGF binding protein gene expression in the rat spleen. In: Regulatory Peptides. 1994 ; Vol. 52, No. 3. pp. 215-226.
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