Role of Estradiol in the Dynamic Control of Tanycyte Plasticity Mediated by Vascular Endothelial Cells in the Median Eminence

Sandrine De Seranno, Xavier D'Anglemont De Tassigny, Cecilia Estrella, Anne Loyens, Sergey Kasparov, Danièle Leroy, Sergio Ojeda, Jean Claude Beauvillain, Vincent Prevot

    Research output: Contribution to journalArticle

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    Abstract

    In the ever-changing physiological context of the neuroendocrine brain, the mechanisms by which cellular events involving neurons, astroglia, and vascular cells are coordinated to bring forth the appropriate neuronal signaling is not yet known but is amenable to examination. In the median eminence of the hypothalamus, endothelial cells are key players in the plasticity of tanycytes (specialized astroglia) and neuroendocrine synapse efficacy. Here we report that estradiol acts on both purified endothelial cells and isolated tanycytes to trigger endothelial-to-glial communication that leads to a sudden and massive retraction of tanycyte processes. The blockade of endothelial nitric oxide synthase by in vitro adenoviral-mediated gene transfer of a dominant-negative form of endothelial nitric oxide synthase abrogates the estradiol-induced tanycyte plasticity mediated by endothelial cells. In parallel, increases in prostaglandin-E2 (PGE2) due to changes in cyclooxygenase (COX)-1 and COX-2 expression induced by the exposure of tanycytes to estradiol promote acute tanycyte plasticity. We also demonstrate by electron microscopy that the administration of PGE2 to median eminence explants induces rapid neuroglial plasticity at the neurovascular junction of neurons that release GnRH (the neuropeptide controlling reproduction). Conversely, preventing local PGE2 synthesis in the median eminence of adult female rats with the COX inhibitor indomethacin impairs the ovarian cycle, a process that requires a pulsatile, coordinated delivery of GnRH into the hypothalamo-hypophyseal portal system. Taken together, our findings show that estradiol controls the dialog between endothelial cells and astroglia to regulate neuroglial plasticity in the neuroendocrine brain.

    Original languageEnglish (US)
    Pages (from-to)1760-1772
    Number of pages13
    JournalEndocrinology
    Volume151
    Issue number4
    DOIs
    StatePublished - Apr 2010

    Fingerprint

    Median Eminence
    Ependymoglial Cells
    Estradiol
    Endothelial Cells
    Dinoprostone
    Astrocytes
    Nitric Oxide Synthase Type III
    Gonadotropin-Releasing Hormone
    Hypothalamo-Hypophyseal System
    Portal System
    Neurons
    Cyclooxygenase 1
    Cyclooxygenase Inhibitors
    Brain
    Cyclooxygenase 2
    Menstrual Cycle
    Neuropeptides
    Neuroglia
    Indomethacin
    Synapses

    ASJC Scopus subject areas

    • Endocrinology

    Cite this

    De Seranno, S., D'Anglemont De Tassigny, X., Estrella, C., Loyens, A., Kasparov, S., Leroy, D., ... Prevot, V. (2010). Role of Estradiol in the Dynamic Control of Tanycyte Plasticity Mediated by Vascular Endothelial Cells in the Median Eminence. Endocrinology, 151(4), 1760-1772. https://doi.org/10.1210/en.2009-0870

    Role of Estradiol in the Dynamic Control of Tanycyte Plasticity Mediated by Vascular Endothelial Cells in the Median Eminence. / De Seranno, Sandrine; D'Anglemont De Tassigny, Xavier; Estrella, Cecilia; Loyens, Anne; Kasparov, Sergey; Leroy, Danièle; Ojeda, Sergio; Beauvillain, Jean Claude; Prevot, Vincent.

    In: Endocrinology, Vol. 151, No. 4, 04.2010, p. 1760-1772.

    Research output: Contribution to journalArticle

    De Seranno, S, D'Anglemont De Tassigny, X, Estrella, C, Loyens, A, Kasparov, S, Leroy, D, Ojeda, S, Beauvillain, JC & Prevot, V 2010, 'Role of Estradiol in the Dynamic Control of Tanycyte Plasticity Mediated by Vascular Endothelial Cells in the Median Eminence', Endocrinology, vol. 151, no. 4, pp. 1760-1772. https://doi.org/10.1210/en.2009-0870
    De Seranno S, D'Anglemont De Tassigny X, Estrella C, Loyens A, Kasparov S, Leroy D et al. Role of Estradiol in the Dynamic Control of Tanycyte Plasticity Mediated by Vascular Endothelial Cells in the Median Eminence. Endocrinology. 2010 Apr;151(4):1760-1772. https://doi.org/10.1210/en.2009-0870
    De Seranno, Sandrine ; D'Anglemont De Tassigny, Xavier ; Estrella, Cecilia ; Loyens, Anne ; Kasparov, Sergey ; Leroy, Danièle ; Ojeda, Sergio ; Beauvillain, Jean Claude ; Prevot, Vincent. / Role of Estradiol in the Dynamic Control of Tanycyte Plasticity Mediated by Vascular Endothelial Cells in the Median Eminence. In: Endocrinology. 2010 ; Vol. 151, No. 4. pp. 1760-1772.
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