Role of electrostatic interactions in binding of thrombin to the fibrinogen γ′ chain

Kristine S. Alexander, Michael G. Fried, David Farrell

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

Thrombin binds to the highly anionic fibrinogen γ′ chain through anion-binding exosite II. This binding profoundly alters thrombin's ability to cleave substrates, including fibrinogen, factor VIII, and PAR1. However, it is unknown whether this interaction is due mainly to general electrostatic complementarity between the γ′ chain and exosite II or if there are critical charged γ′ chain residues involved. We therefore systematically determined the contribution of negatively charged amino acids in the γ′ chain, both individually and collectively, to thrombin binding affinity. Surface plasmon resonance binding experiments were performed using immobilized γ′ chain peptides with charged-to-uncharged amino acid substitutions, i.e., Asp to Asn, Glu to Gln, and pTyr to Tyr. Individually, the substitution of uncharged for charged amino acids resulted in only minor changes in binding affinity, with a maximal change in Kd from 0.440 to 0.705 μM for the Asp419Asn substitution. However, substitution of all three charged amino acids in a conserved β-turn that is predicted to contact thrombin, pTyr418Tyr, Asp419Asn, and pTyr422Tyr, resulted in the loss of measurable binding, as did substitution of all the flanking charged amino acids. In addition, the binding of the γ′ chain to thrombin was weakened in a dose-dependent manner with increasing NaCl concentration, resulting in a net loss of three or four ion pairs between thrombin and the γ′ chain. Therefore, although each of the individual charges in the γ′ chain contributes only incrementally to the overall binding affinity, the ensemble of the combined charges plays a profound role in the thrombin-γ′ chain interactions.

Original languageEnglish (US)
Pages (from-to)3445-3450
Number of pages6
JournalBiochemistry
Volume51
Issue number16
DOIs
StatePublished - Apr 24 2012

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Coulomb interactions
Static Electricity
Thrombin
Fibrinogen
Substitution reactions
Amino Acids
Surface Plasmon Resonance
Factor VIII
Surface plasmon resonance
Amino Acid Substitution
Anions
Electrostatics
Ions
Peptides
Substrates
Experiments

ASJC Scopus subject areas

  • Biochemistry

Cite this

Role of electrostatic interactions in binding of thrombin to the fibrinogen γ′ chain. / Alexander, Kristine S.; Fried, Michael G.; Farrell, David.

In: Biochemistry, Vol. 51, No. 16, 24.04.2012, p. 3445-3450.

Research output: Contribution to journalArticle

Alexander, Kristine S. ; Fried, Michael G. ; Farrell, David. / Role of electrostatic interactions in binding of thrombin to the fibrinogen γ′ chain. In: Biochemistry. 2012 ; Vol. 51, No. 16. pp. 3445-3450.
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