Role of actin cortex in the subplasmalemmal transport of secretory granules in PC-12 cells

Thorsten Lang, Irene Wacker, Ilse Wunderlich, Alexander Rohrbach, Günter Giese, Thierry Soldati, Wolfhard Almers

Research output: Contribution to journalArticle

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Abstract

In neuroendocrine PC-12 cells, evanescent-field fluorescence microsopy was used to track motions of green fluorescent protein (GFP)-labeled actin or GFP-labeled secretory granules in a thin layer of cytoplasm where cells adhered to glass. The layer contained abundant filamentous actin (F-actin) locally condensed into stress fibers. More than 90% of the granules imaged lay within the F-actin layer. One-third of the granules did not move detectably, while two-thirds moved randomly; the average diffusion coefficient was 23 x 10-4 μm2/s. A small minority (2+ and replacing ATP with AMP-PNP. Mobility was blocked also when F-actin was stabilized with phalloidin, and was diminished when the actin cortex was degraded with latrunculin B. We conclude that the movement of granules requires metabolic energy, and that it is mediated as well as limited by the actin cortex. Opposing actions of the actin cortex on mobility may explain why its degradation has variable effects on secretion.

Original languageEnglish (US)
Pages (from-to)2863-2877
Number of pages15
JournalBiophysical Journal
Volume78
Issue number6
StatePublished - Jun 2000

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Secretory Vesicles
Actins
Green Fluorescent Proteins
Adenylyl Imidodiphosphate
Phalloidine
Stress Fibers
Glass
Cytoplasm
Adenosine Triphosphate
Fluorescence

ASJC Scopus subject areas

  • Biophysics

Cite this

Lang, T., Wacker, I., Wunderlich, I., Rohrbach, A., Giese, G., Soldati, T., & Almers, W. (2000). Role of actin cortex in the subplasmalemmal transport of secretory granules in PC-12 cells. Biophysical Journal, 78(6), 2863-2877.

Role of actin cortex in the subplasmalemmal transport of secretory granules in PC-12 cells. / Lang, Thorsten; Wacker, Irene; Wunderlich, Ilse; Rohrbach, Alexander; Giese, Günter; Soldati, Thierry; Almers, Wolfhard.

In: Biophysical Journal, Vol. 78, No. 6, 06.2000, p. 2863-2877.

Research output: Contribution to journalArticle

Lang, T, Wacker, I, Wunderlich, I, Rohrbach, A, Giese, G, Soldati, T & Almers, W 2000, 'Role of actin cortex in the subplasmalemmal transport of secretory granules in PC-12 cells', Biophysical Journal, vol. 78, no. 6, pp. 2863-2877.
Lang T, Wacker I, Wunderlich I, Rohrbach A, Giese G, Soldati T et al. Role of actin cortex in the subplasmalemmal transport of secretory granules in PC-12 cells. Biophysical Journal. 2000 Jun;78(6):2863-2877.
Lang, Thorsten ; Wacker, Irene ; Wunderlich, Ilse ; Rohrbach, Alexander ; Giese, Günter ; Soldati, Thierry ; Almers, Wolfhard. / Role of actin cortex in the subplasmalemmal transport of secretory granules in PC-12 cells. In: Biophysical Journal. 2000 ; Vol. 78, No. 6. pp. 2863-2877.
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