Role of AbrB in Spo0A- and Spo0B-dependent utilization of a sporulation promoter in Bacillus subtilis

Peter Zuber, R. Losick

Research output: Contribution to journalArticle

168 Citations (Scopus)

Abstract

Transcription of the Bacillus subtilis gene spoVG is induced at the onset of sporulation and is dependent on the products of the stage-0 regulatory genes spo0A, spo0B, and spo0H. We show here that the dependence of spoVG transcription on Spo0A and Spo0B (but not Spo0H) can be bypassed by a mutation at abrB, a previously identified locus at which mutations that suppress some of the phenotypes of spo0A are often located, or by a cis-acting mutations within the spoVG promoter. To explain the epistatis of abrB to spo0A and spo0B mutations, we propose that AbrB acts, directly or indirectly, to block transcription of spoVG and that Spo0A and Spo0B cause inactivation of the abrB gene product(s). Spo0A-Spo0B-dependent inactivation of AbrB could be general explanation for the pleiotropic effect of spo0A and spo0B mutations on B. subtilis gene expression.

Original languageEnglish (US)
Pages (from-to)2223-2230
Number of pages8
JournalJournal of Bacteriology
Volume169
Issue number5
StatePublished - 1987
Externally publishedYes

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Bacillus subtilis
Mutation
Gene Silencing
Regulator Genes
Phenotype
Gene Expression
Genes

ASJC Scopus subject areas

  • Applied Microbiology and Biotechnology
  • Immunology

Cite this

Role of AbrB in Spo0A- and Spo0B-dependent utilization of a sporulation promoter in Bacillus subtilis. / Zuber, Peter; Losick, R.

In: Journal of Bacteriology, Vol. 169, No. 5, 1987, p. 2223-2230.

Research output: Contribution to journalArticle

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N2 - Transcription of the Bacillus subtilis gene spoVG is induced at the onset of sporulation and is dependent on the products of the stage-0 regulatory genes spo0A, spo0B, and spo0H. We show here that the dependence of spoVG transcription on Spo0A and Spo0B (but not Spo0H) can be bypassed by a mutation at abrB, a previously identified locus at which mutations that suppress some of the phenotypes of spo0A are often located, or by a cis-acting mutations within the spoVG promoter. To explain the epistatis of abrB to spo0A and spo0B mutations, we propose that AbrB acts, directly or indirectly, to block transcription of spoVG and that Spo0A and Spo0B cause inactivation of the abrB gene product(s). Spo0A-Spo0B-dependent inactivation of AbrB could be general explanation for the pleiotropic effect of spo0A and spo0B mutations on B. subtilis gene expression.

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