Role for lipid signaling and the cell integrity MAP kinase cascade in yeast septum biogenesis

Sabina Tahirovic, Markus Schorr, Angela Then, Jürgen Berger, Heinz Schwarz, Peter Mayinger

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

Polarized deposition of chitin at the bud neck is essential for cell separation in yeast. Chitin septum biogenesis is catalyzed by two distinct chitin synthase activities encoded by the CHS2 and CHS3 genes. The phosphoinositide phosphatase Sac1p is required for proper trafficking of the Chs3p chitin synthase. sac1 mutants also display a severe synthetic growth defect, with mutations in the SLT2 gene which encodes a MAP kinase involved in cell integrity. We characterized the defect that underlies this genetic interaction and found that sac1Δ slt2Δ cells arrest as large-budded cells because they fail to separate at the end of mitosis. This inability to complete cell division appears to be caused by an increased deposition of chitin at the septum area and correlates with a mislocalized accumulation of the Chs2p chitin synthase at the cell periphery. Our data therefore indicate that Sac1p and Slt2p have synergistic roles in regulating chitin septum biogenesis.

Original languageEnglish (US)
Pages (from-to)71-78
Number of pages8
JournalCurrent Genetics
Volume43
Issue number2
StatePublished - May 1 2003
Externally publishedYes

Fingerprint

Chitin
MAP Kinase Signaling System
Chitin Synthase
Yeasts
Lipids
Cell Separation
Mitosis
Cell Division
Genes
Phosphotransferases
Mutation
Growth

Keywords

  • Cell separation
  • MAP kinase
  • Phosphoinositide phosphatase
  • Sac1p
  • Slt2p

ASJC Scopus subject areas

  • Genetics

Cite this

Tahirovic, S., Schorr, M., Then, A., Berger, J., Schwarz, H., & Mayinger, P. (2003). Role for lipid signaling and the cell integrity MAP kinase cascade in yeast septum biogenesis. Current Genetics, 43(2), 71-78.

Role for lipid signaling and the cell integrity MAP kinase cascade in yeast septum biogenesis. / Tahirovic, Sabina; Schorr, Markus; Then, Angela; Berger, Jürgen; Schwarz, Heinz; Mayinger, Peter.

In: Current Genetics, Vol. 43, No. 2, 01.05.2003, p. 71-78.

Research output: Contribution to journalArticle

Tahirovic, S, Schorr, M, Then, A, Berger, J, Schwarz, H & Mayinger, P 2003, 'Role for lipid signaling and the cell integrity MAP kinase cascade in yeast septum biogenesis', Current Genetics, vol. 43, no. 2, pp. 71-78.
Tahirovic, Sabina ; Schorr, Markus ; Then, Angela ; Berger, Jürgen ; Schwarz, Heinz ; Mayinger, Peter. / Role for lipid signaling and the cell integrity MAP kinase cascade in yeast septum biogenesis. In: Current Genetics. 2003 ; Vol. 43, No. 2. pp. 71-78.
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