Rod Cell-Specific Antigens in Retinoblastoma

Tamara Vrabec, Violeta Arbizo, Grazyna Adamus, J. Hugh Mcdowell, Paul A. Hargrave, Larry A. Donoso

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

We investigated rhodopsin immunoreactivity in five well-differentiated retinoblastomas using a panel of monoclonal antibodies directed against specific antigenic sites in the amino- and carboxyl-terminal portions of rhodopsin. All five monoclonal antibodies bound to the rod cell outer segment of nontumorous retina in all 10% formaldehyde solution-fixed, paraffin-embedded tissue sections. A characteristic “halo” cell surface staining pattern was observed in four (80%) of five tumors treated with two monoclonal antibodies, B6-30 (rhodopsin amino-terminal specific) and K16-107 (rhodopsin carboxyl-terminal specific). In each case, the staining pattern was limited to well-differentiated areas of the tumor containing Flexner-Wintersteiner rosettes or fleurettes. One tumor was not stained by any monoclonal antibody, whereas all monoclonal antibodies stained the rod cell outer segments of nontumorous retina. Our studies indicate that selected retinoblastomas may be differentiated along a rod photoreceptorlike cell lineage.

Original languageEnglish (US)
Pages (from-to)1061-1063
Number of pages3
JournalArchives of Ophthalmology
Volume107
Issue number7
DOIs
StatePublished - 1989
Externally publishedYes

Fingerprint

Retinoblastoma
Rhodopsin
Monoclonal Antibodies
Antigens
Rod Cell Outer Segment
Retina
Staining and Labeling
Neoplasms
Cell Lineage
Paraffin
Formaldehyde

ASJC Scopus subject areas

  • Ophthalmology

Cite this

Vrabec, T., Arbizo, V., Adamus, G., Mcdowell, J. H., Hargrave, P. A., & Donoso, L. A. (1989). Rod Cell-Specific Antigens in Retinoblastoma. Archives of Ophthalmology, 107(7), 1061-1063. https://doi.org/10.1001/archopht.1989.01070020123044

Rod Cell-Specific Antigens in Retinoblastoma. / Vrabec, Tamara; Arbizo, Violeta; Adamus, Grazyna; Mcdowell, J. Hugh; Hargrave, Paul A.; Donoso, Larry A.

In: Archives of Ophthalmology, Vol. 107, No. 7, 1989, p. 1061-1063.

Research output: Contribution to journalArticle

Vrabec, T, Arbizo, V, Adamus, G, Mcdowell, JH, Hargrave, PA & Donoso, LA 1989, 'Rod Cell-Specific Antigens in Retinoblastoma', Archives of Ophthalmology, vol. 107, no. 7, pp. 1061-1063. https://doi.org/10.1001/archopht.1989.01070020123044
Vrabec, Tamara ; Arbizo, Violeta ; Adamus, Grazyna ; Mcdowell, J. Hugh ; Hargrave, Paul A. ; Donoso, Larry A. / Rod Cell-Specific Antigens in Retinoblastoma. In: Archives of Ophthalmology. 1989 ; Vol. 107, No. 7. pp. 1061-1063.
@article{43f460b48f0347dcb5d178fe78b6e45c,
title = "Rod Cell-Specific Antigens in Retinoblastoma",
abstract = "We investigated rhodopsin immunoreactivity in five well-differentiated retinoblastomas using a panel of monoclonal antibodies directed against specific antigenic sites in the amino- and carboxyl-terminal portions of rhodopsin. All five monoclonal antibodies bound to the rod cell outer segment of nontumorous retina in all 10{\%} formaldehyde solution-fixed, paraffin-embedded tissue sections. A characteristic “halo” cell surface staining pattern was observed in four (80{\%}) of five tumors treated with two monoclonal antibodies, B6-30 (rhodopsin amino-terminal specific) and K16-107 (rhodopsin carboxyl-terminal specific). In each case, the staining pattern was limited to well-differentiated areas of the tumor containing Flexner-Wintersteiner rosettes or fleurettes. One tumor was not stained by any monoclonal antibody, whereas all monoclonal antibodies stained the rod cell outer segments of nontumorous retina. Our studies indicate that selected retinoblastomas may be differentiated along a rod photoreceptorlike cell lineage.",
author = "Tamara Vrabec and Violeta Arbizo and Grazyna Adamus and Mcdowell, {J. Hugh} and Hargrave, {Paul A.} and Donoso, {Larry A.}",
year = "1989",
doi = "10.1001/archopht.1989.01070020123044",
language = "English (US)",
volume = "107",
pages = "1061--1063",
journal = "JAMA Ophthalmology",
issn = "2168-6165",
publisher = "American Medical Association",
number = "7",

}

TY - JOUR

T1 - Rod Cell-Specific Antigens in Retinoblastoma

AU - Vrabec, Tamara

AU - Arbizo, Violeta

AU - Adamus, Grazyna

AU - Mcdowell, J. Hugh

AU - Hargrave, Paul A.

AU - Donoso, Larry A.

PY - 1989

Y1 - 1989

N2 - We investigated rhodopsin immunoreactivity in five well-differentiated retinoblastomas using a panel of monoclonal antibodies directed against specific antigenic sites in the amino- and carboxyl-terminal portions of rhodopsin. All five monoclonal antibodies bound to the rod cell outer segment of nontumorous retina in all 10% formaldehyde solution-fixed, paraffin-embedded tissue sections. A characteristic “halo” cell surface staining pattern was observed in four (80%) of five tumors treated with two monoclonal antibodies, B6-30 (rhodopsin amino-terminal specific) and K16-107 (rhodopsin carboxyl-terminal specific). In each case, the staining pattern was limited to well-differentiated areas of the tumor containing Flexner-Wintersteiner rosettes or fleurettes. One tumor was not stained by any monoclonal antibody, whereas all monoclonal antibodies stained the rod cell outer segments of nontumorous retina. Our studies indicate that selected retinoblastomas may be differentiated along a rod photoreceptorlike cell lineage.

AB - We investigated rhodopsin immunoreactivity in five well-differentiated retinoblastomas using a panel of monoclonal antibodies directed against specific antigenic sites in the amino- and carboxyl-terminal portions of rhodopsin. All five monoclonal antibodies bound to the rod cell outer segment of nontumorous retina in all 10% formaldehyde solution-fixed, paraffin-embedded tissue sections. A characteristic “halo” cell surface staining pattern was observed in four (80%) of five tumors treated with two monoclonal antibodies, B6-30 (rhodopsin amino-terminal specific) and K16-107 (rhodopsin carboxyl-terminal specific). In each case, the staining pattern was limited to well-differentiated areas of the tumor containing Flexner-Wintersteiner rosettes or fleurettes. One tumor was not stained by any monoclonal antibody, whereas all monoclonal antibodies stained the rod cell outer segments of nontumorous retina. Our studies indicate that selected retinoblastomas may be differentiated along a rod photoreceptorlike cell lineage.

UR - http://www.scopus.com/inward/record.url?scp=0024403926&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0024403926&partnerID=8YFLogxK

U2 - 10.1001/archopht.1989.01070020123044

DO - 10.1001/archopht.1989.01070020123044

M3 - Article

C2 - 2473730

AN - SCOPUS:0024403926

VL - 107

SP - 1061

EP - 1063

JO - JAMA Ophthalmology

JF - JAMA Ophthalmology

SN - 2168-6165

IS - 7

ER -