Robust expansion of human hepatocytes in Fah-/-/Rag2 -/-/Il2rg-/- mice

Hisaya Azuma, Nicole Paulk, Aarati Ranade, Craig Dorrell, Muhsen Al-Dhalimy, Ewa Ellis, Stephen Strom, Mark A. Kay, Milton Finegold, Markus Grompe

Research output: Contribution to journalArticle

458 Scopus citations

Abstract

Mice that could be highly repopulated with human hepatocytes would have many potential uses in drug development and research applications. The best available model of liver humanization, the uroplasminogen-activator transgenic model, has major practical limitations. To provide a broadly useful hepatic xenorepopulation system, we generated severely immunodeficient, fumarylacetoacetate hydrolase (Fah)-deficient mice. After pretreatment with a urokinase-expressing adenovirus, these animals could be highly engrafted (up to 90%) with human hepatocytes from multiple sources, including liver biopsies. Furthermore, human cells could be serially transplanted from primary donors and repopulate the liver for at least four sequential rounds. The expanded cells displayed typical human drug metabolism. This system provides a robust platform to produce high-quality human hepatocytes for tissue culture. It may also be useful for testing the toxicity of drug metabolites and for evaluating pathogens dependent on human liver cells for replication.

Original languageEnglish (US)
Pages (from-to)903-910
Number of pages8
JournalNature biotechnology
Volume25
Issue number8
DOIs
StatePublished - Aug 1 2007

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ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Applied Microbiology and Biotechnology
  • Molecular Medicine
  • Biomedical Engineering

Cite this

Azuma, H., Paulk, N., Ranade, A., Dorrell, C., Al-Dhalimy, M., Ellis, E., Strom, S., Kay, M. A., Finegold, M., & Grompe, M. (2007). Robust expansion of human hepatocytes in Fah-/-/Rag2 -/-/Il2rg-/- mice. Nature biotechnology, 25(8), 903-910. https://doi.org/10.1038/nbt1326