RNases J1 and J2 are critical pleiotropic regulators in Streptococcus mutans

Xi Chen, Nan Liu, Sharukh Khajotia, Fengxia Qi, Justin Merritt

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

In recent years, it has become increasingly evident that post-transcriptional control mechanisms are the principal source of gene regulation for a large number of prokaryotic genetic pathways, particularly those involved in virulence and environmental adaptation. Post-transcriptional regulation is largely governed by RNA stability, which itself is determined by target accessibility to RNase degradation. In most Firmicutes species, mRNA stability is strongly impacted by the activity of two recently discovered RNases referred to as RNase J1 and RNase J2. Little is known about RNase J1 function in bacteria and even less is known about RNase J2. In the current study, we mutated both RNase J orthologues in Streptococcus mutans to determine their functional roles in the cell. Single and double RNase J mutants were viable, but grew very slowly on agar plates. All of the mutants shared substantial defects in growth, morphology, acid tolerance, natural competence and biofilm formation. However, most of these defects were more severe in the RNase J2 mutant. Phenotypic suppression results also implicate a role for RNase J2 as a regulator of RNase J1 function. Unlike Bacillus subtilis, RNase J2 is a major pleiotropic regulator in S. mutans, which indicates some fundamental differences from B. subtilis in global gene regulation. Key conserved residues among the RNase J2 orthologues of lactic acid bacteria may hint at a greater role for RNase J2 in these species.

Original languageEnglish (US)
Pages (from-to)797-806
Number of pages10
JournalMicrobiology (Reading, England)
Volume161
DOIs
StatePublished - Apr 1 2015
Externally publishedYes

Fingerprint

Streptococcus mutans
Ribonucleases
RNA Stability
Bacillus subtilis
Bacteria
Biofilms
Mental Competency
Genes
Agar
Virulence
Lactic Acid

ASJC Scopus subject areas

  • Microbiology

Cite this

RNases J1 and J2 are critical pleiotropic regulators in Streptococcus mutans. / Chen, Xi; Liu, Nan; Khajotia, Sharukh; Qi, Fengxia; Merritt, Justin.

In: Microbiology (Reading, England), Vol. 161, 01.04.2015, p. 797-806.

Research output: Contribution to journalArticle

Chen, Xi ; Liu, Nan ; Khajotia, Sharukh ; Qi, Fengxia ; Merritt, Justin. / RNases J1 and J2 are critical pleiotropic regulators in Streptococcus mutans. In: Microbiology (Reading, England). 2015 ; Vol. 161. pp. 797-806.
@article{3f9d9b4d8be4497ca22ad7c9c71925ff,
title = "RNases J1 and J2 are critical pleiotropic regulators in Streptococcus mutans",
abstract = "In recent years, it has become increasingly evident that post-transcriptional control mechanisms are the principal source of gene regulation for a large number of prokaryotic genetic pathways, particularly those involved in virulence and environmental adaptation. Post-transcriptional regulation is largely governed by RNA stability, which itself is determined by target accessibility to RNase degradation. In most Firmicutes species, mRNA stability is strongly impacted by the activity of two recently discovered RNases referred to as RNase J1 and RNase J2. Little is known about RNase J1 function in bacteria and even less is known about RNase J2. In the current study, we mutated both RNase J orthologues in Streptococcus mutans to determine their functional roles in the cell. Single and double RNase J mutants were viable, but grew very slowly on agar plates. All of the mutants shared substantial defects in growth, morphology, acid tolerance, natural competence and biofilm formation. However, most of these defects were more severe in the RNase J2 mutant. Phenotypic suppression results also implicate a role for RNase J2 as a regulator of RNase J1 function. Unlike Bacillus subtilis, RNase J2 is a major pleiotropic regulator in S. mutans, which indicates some fundamental differences from B. subtilis in global gene regulation. Key conserved residues among the RNase J2 orthologues of lactic acid bacteria may hint at a greater role for RNase J2 in these species.",
author = "Xi Chen and Nan Liu and Sharukh Khajotia and Fengxia Qi and Justin Merritt",
year = "2015",
month = "4",
day = "1",
doi = "10.1099/mic.0.000039",
language = "English (US)",
volume = "161",
pages = "797--806",
journal = "Microbiology",
issn = "1350-0872",
publisher = "Society for General Microbiology",

}

TY - JOUR

T1 - RNases J1 and J2 are critical pleiotropic regulators in Streptococcus mutans

AU - Chen, Xi

AU - Liu, Nan

AU - Khajotia, Sharukh

AU - Qi, Fengxia

AU - Merritt, Justin

PY - 2015/4/1

Y1 - 2015/4/1

N2 - In recent years, it has become increasingly evident that post-transcriptional control mechanisms are the principal source of gene regulation for a large number of prokaryotic genetic pathways, particularly those involved in virulence and environmental adaptation. Post-transcriptional regulation is largely governed by RNA stability, which itself is determined by target accessibility to RNase degradation. In most Firmicutes species, mRNA stability is strongly impacted by the activity of two recently discovered RNases referred to as RNase J1 and RNase J2. Little is known about RNase J1 function in bacteria and even less is known about RNase J2. In the current study, we mutated both RNase J orthologues in Streptococcus mutans to determine their functional roles in the cell. Single and double RNase J mutants were viable, but grew very slowly on agar plates. All of the mutants shared substantial defects in growth, morphology, acid tolerance, natural competence and biofilm formation. However, most of these defects were more severe in the RNase J2 mutant. Phenotypic suppression results also implicate a role for RNase J2 as a regulator of RNase J1 function. Unlike Bacillus subtilis, RNase J2 is a major pleiotropic regulator in S. mutans, which indicates some fundamental differences from B. subtilis in global gene regulation. Key conserved residues among the RNase J2 orthologues of lactic acid bacteria may hint at a greater role for RNase J2 in these species.

AB - In recent years, it has become increasingly evident that post-transcriptional control mechanisms are the principal source of gene regulation for a large number of prokaryotic genetic pathways, particularly those involved in virulence and environmental adaptation. Post-transcriptional regulation is largely governed by RNA stability, which itself is determined by target accessibility to RNase degradation. In most Firmicutes species, mRNA stability is strongly impacted by the activity of two recently discovered RNases referred to as RNase J1 and RNase J2. Little is known about RNase J1 function in bacteria and even less is known about RNase J2. In the current study, we mutated both RNase J orthologues in Streptococcus mutans to determine their functional roles in the cell. Single and double RNase J mutants were viable, but grew very slowly on agar plates. All of the mutants shared substantial defects in growth, morphology, acid tolerance, natural competence and biofilm formation. However, most of these defects were more severe in the RNase J2 mutant. Phenotypic suppression results also implicate a role for RNase J2 as a regulator of RNase J1 function. Unlike Bacillus subtilis, RNase J2 is a major pleiotropic regulator in S. mutans, which indicates some fundamental differences from B. subtilis in global gene regulation. Key conserved residues among the RNase J2 orthologues of lactic acid bacteria may hint at a greater role for RNase J2 in these species.

UR - http://www.scopus.com/inward/record.url?scp=85017330945&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85017330945&partnerID=8YFLogxK

U2 - 10.1099/mic.0.000039

DO - 10.1099/mic.0.000039

M3 - Article

C2 - 25635274

AN - SCOPUS:85017330945

VL - 161

SP - 797

EP - 806

JO - Microbiology

JF - Microbiology

SN - 1350-0872

ER -