RNA type III secretion signals that require Hfq

George S. Niemann, Roslyn N. Brown, Ivy T. Mushamiri, Nhu T. Nguyen, Rukayat Taiwo, Afke Stufkens, Richard D. Smith, Joshua N. Adkins, Jason E. McDermott, Fred Heffron

    Research output: Contribution to journalArticle

    13 Citations (Scopus)

    Abstract

    Salmonella virulence is largely mediated by two type III secretion systems (T3SS) that deliver effector proteins from the bacterium to a host cell; however, the secretion signal is poorly defined. Effector N termini are thought to contain the signal, but they lack homology, possess no identifiable motif, and adopt intrinsically disordered structures. Alternative studies suggest that RNA-encoded signals may also be recognized and that they can be located in the 5= untranslated leader sequence. We began our study by establishing the minimum sequence required for reporter translocation. Untranslated leader sequences predicted from 42 different Salmonella effector proteins were fused to the adenylate cyclase reporter (CyaA=), and each of them was tested for protein injection into J774 macrophages. RNA sequences derived from five effectors, gtgA, cigR, gogB, sseL, and steD, were sufficient for CyaA= translocation into host cells. To determine the mechanism of signal recognition, we identified proteins that bound specifically to the gtgA RNA. One of the unique proteins identified was Hfq. Hfq had no effect upon the translocation of full-length CigR and SteD, but injection of intact GtgA, GogB, and SseL was abolished in an hfq mutant, confirming the importance of Hfq. Our results demonstrated that the Salmonella pathogenicity island 2 (SPI-2) T3SS assembled into a functional apparatus independently of Hfq. Since particular effectors required Hfq for translocation, Hfq-RNA complexes may participate in signal recognition.

    Original languageEnglish (US)
    Pages (from-to)2119-2125
    Number of pages7
    JournalJournal of Bacteriology
    Volume195
    Issue number10
    DOIs
    StatePublished - May 2013

    Fingerprint

    RNA
    Salmonella
    Proteins
    Genomic Islands
    Injections
    Adenylyl Cyclases
    Virulence
    Macrophages
    Bacteria

    ASJC Scopus subject areas

    • Microbiology
    • Molecular Biology

    Cite this

    Niemann, G. S., Brown, R. N., Mushamiri, I. T., Nguyen, N. T., Taiwo, R., Stufkens, A., ... Heffron, F. (2013). RNA type III secretion signals that require Hfq. Journal of Bacteriology, 195(10), 2119-2125. https://doi.org/10.1128/JB.00024-13

    RNA type III secretion signals that require Hfq. / Niemann, George S.; Brown, Roslyn N.; Mushamiri, Ivy T.; Nguyen, Nhu T.; Taiwo, Rukayat; Stufkens, Afke; Smith, Richard D.; Adkins, Joshua N.; McDermott, Jason E.; Heffron, Fred.

    In: Journal of Bacteriology, Vol. 195, No. 10, 05.2013, p. 2119-2125.

    Research output: Contribution to journalArticle

    Niemann, GS, Brown, RN, Mushamiri, IT, Nguyen, NT, Taiwo, R, Stufkens, A, Smith, RD, Adkins, JN, McDermott, JE & Heffron, F 2013, 'RNA type III secretion signals that require Hfq', Journal of Bacteriology, vol. 195, no. 10, pp. 2119-2125. https://doi.org/10.1128/JB.00024-13
    Niemann GS, Brown RN, Mushamiri IT, Nguyen NT, Taiwo R, Stufkens A et al. RNA type III secretion signals that require Hfq. Journal of Bacteriology. 2013 May;195(10):2119-2125. https://doi.org/10.1128/JB.00024-13
    Niemann, George S. ; Brown, Roslyn N. ; Mushamiri, Ivy T. ; Nguyen, Nhu T. ; Taiwo, Rukayat ; Stufkens, Afke ; Smith, Richard D. ; Adkins, Joshua N. ; McDermott, Jason E. ; Heffron, Fred. / RNA type III secretion signals that require Hfq. In: Journal of Bacteriology. 2013 ; Vol. 195, No. 10. pp. 2119-2125.
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