RNA directed DNA polymerase activity in Greene hamster melanoma

T. W. Reid, T. L. Darling, P. Russell, Daniel Albert

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

As part of a biochemical search for a virus in melanomas preliminary kinetic studies with RNA directed DNA polymerase from avian myeloblastosis virus showed that thymidine labeled triphosphate (TTP) stimulates the enzyme. This stimulatory effect was used as an additional probe in detecting RNA directed DNA polymerase activity in Greene hamster melanoma in vivo. This enzyme activity, however, was not found in the Greene hamster melanoma cells growing in tissue culture under ordinary conditions. When the cells were treated with BUDR the enzyme could be demonstrated and the stimulating effect of TTP was observed.

Original languageEnglish (US)
Pages (from-to)485-491
Number of pages7
JournalYale Journal of Biology and Medicine
Volume46
Issue number5
StatePublished - Dec 1 1973
Externally publishedYes

Fingerprint

RNA-Directed DNA Polymerase
Viruses
Cricetinae
Thymidine
Melanoma
Tissue culture
Enzyme activity
Enzymes
Avian Myeloblastosis Virus
Kinetics
triphosphoric acid
thymidine 5'-triphosphate

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

RNA directed DNA polymerase activity in Greene hamster melanoma. / Reid, T. W.; Darling, T. L.; Russell, P.; Albert, Daniel.

In: Yale Journal of Biology and Medicine, Vol. 46, No. 5, 01.12.1973, p. 485-491.

Research output: Contribution to journalArticle

Reid, TW, Darling, TL, Russell, P & Albert, D 1973, 'RNA directed DNA polymerase activity in Greene hamster melanoma', Yale Journal of Biology and Medicine, vol. 46, no. 5, pp. 485-491.
Reid, T. W. ; Darling, T. L. ; Russell, P. ; Albert, Daniel. / RNA directed DNA polymerase activity in Greene hamster melanoma. In: Yale Journal of Biology and Medicine. 1973 ; Vol. 46, No. 5. pp. 485-491.
@article{9b5662c73ab8480ab69cc24a315d10d3,
title = "RNA directed DNA polymerase activity in Greene hamster melanoma",
abstract = "As part of a biochemical search for a virus in melanomas preliminary kinetic studies with RNA directed DNA polymerase from avian myeloblastosis virus showed that thymidine labeled triphosphate (TTP) stimulates the enzyme. This stimulatory effect was used as an additional probe in detecting RNA directed DNA polymerase activity in Greene hamster melanoma in vivo. This enzyme activity, however, was not found in the Greene hamster melanoma cells growing in tissue culture under ordinary conditions. When the cells were treated with BUDR the enzyme could be demonstrated and the stimulating effect of TTP was observed.",
author = "Reid, {T. W.} and Darling, {T. L.} and P. Russell and Daniel Albert",
year = "1973",
month = "12",
day = "1",
language = "English (US)",
volume = "46",
pages = "485--491",
journal = "Yale Journal of Biology and Medicine",
issn = "0044-0086",
publisher = "Yale Journal of Biology and Medicine Inc.",
number = "5",

}

TY - JOUR

T1 - RNA directed DNA polymerase activity in Greene hamster melanoma

AU - Reid, T. W.

AU - Darling, T. L.

AU - Russell, P.

AU - Albert, Daniel

PY - 1973/12/1

Y1 - 1973/12/1

N2 - As part of a biochemical search for a virus in melanomas preliminary kinetic studies with RNA directed DNA polymerase from avian myeloblastosis virus showed that thymidine labeled triphosphate (TTP) stimulates the enzyme. This stimulatory effect was used as an additional probe in detecting RNA directed DNA polymerase activity in Greene hamster melanoma in vivo. This enzyme activity, however, was not found in the Greene hamster melanoma cells growing in tissue culture under ordinary conditions. When the cells were treated with BUDR the enzyme could be demonstrated and the stimulating effect of TTP was observed.

AB - As part of a biochemical search for a virus in melanomas preliminary kinetic studies with RNA directed DNA polymerase from avian myeloblastosis virus showed that thymidine labeled triphosphate (TTP) stimulates the enzyme. This stimulatory effect was used as an additional probe in detecting RNA directed DNA polymerase activity in Greene hamster melanoma in vivo. This enzyme activity, however, was not found in the Greene hamster melanoma cells growing in tissue culture under ordinary conditions. When the cells were treated with BUDR the enzyme could be demonstrated and the stimulating effect of TTP was observed.

UR - http://www.scopus.com/inward/record.url?scp=0015754739&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0015754739&partnerID=8YFLogxK

M3 - Article

VL - 46

SP - 485

EP - 491

JO - Yale Journal of Biology and Medicine

JF - Yale Journal of Biology and Medicine

SN - 0044-0086

IS - 5

ER -