RNA-dependent DNA-polymerase activity in human tumors

Ted W. Reid, Daniel M. Albert

Research output: Contribution to journalArticle

19 Scopus citations

Abstract

RNA-dependent DNA-polymerase activity is present in specimens of freshly excised human medulloblastoma. This activity is demonstrated in the cell homogenates and activity remains in the supernatant fluid at 30,000g after 30 minutes and pellets at 100,000g in 2 hrs. Detergent is necessary to elicit significant activity. The enzyme activity was determined using a poly(A)·oligo(dT) template. Retinoblastoma, neuroblastoma and ocular malignant melanoma also showed activity but in much lower amounts than medulloblastoma. Normal human orbital connective tissue, cerebellum and retina, similarly studied, failed to show activity with this template. Thus, by these methods a RNA-dependent DNA-polymerase activity is demonstrated in human tumors, but not in the corresponding normal tissues.

Original languageEnglish (US)
Pages (from-to)383-390
Number of pages8
JournalBiochemical and Biophysical Research Communications
Volume46
Issue number2
DOIs
StatePublished - Jan 31 1972

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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