RNA-dependent DNA-polymerase activity in human tumors

Ted W. Reid, Daniel Albert

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

RNA-dependent DNA-polymerase activity is present in specimens of freshly excised human medulloblastoma. This activity is demonstrated in the cell homogenates and activity remains in the supernatant fluid at 30,000g after 30 minutes and pellets at 100,000g in 2 hrs. Detergent is necessary to elicit significant activity. The enzyme activity was determined using a poly(A)·oligo(dT) template. Retinoblastoma, neuroblastoma and ocular malignant melanoma also showed activity but in much lower amounts than medulloblastoma. Normal human orbital connective tissue, cerebellum and retina, similarly studied, failed to show activity with this template. Thus, by these methods a RNA-dependent DNA-polymerase activity is demonstrated in human tumors, but not in the corresponding normal tissues.

Original languageEnglish (US)
Pages (from-to)383-390
Number of pages8
JournalBiochemical and Biophysical Research Communications
Volume46
Issue number2
DOIs
StatePublished - Jan 31 1972
Externally publishedYes

Fingerprint

RNA-Directed DNA Polymerase
Human Activities
Tumors
Medulloblastoma
Tissue
Poly A
Enzyme activity
Detergents
Neoplasms
Retinoblastoma
Neuroblastoma
Connective Tissue
Cerebellum
Fluids
Retina
Melanoma
Enzymes
oligo (dT)

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

RNA-dependent DNA-polymerase activity in human tumors. / Reid, Ted W.; Albert, Daniel.

In: Biochemical and Biophysical Research Communications, Vol. 46, No. 2, 31.01.1972, p. 383-390.

Research output: Contribution to journalArticle

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