TY - JOUR
T1 - RNA-dependent DNA-polymerase activity in human tumors
AU - Reid, Ted W.
AU - Albert, Daniel M.
N1 - Funding Information:
We thank Dr. Alan S. Rabson for his help in formulating this study and subsequent advice and. Dr. Marvin L. Sears for his suggestions and support. Drs. Peter Langyel, Jeffery Schlom. David Ward, Mr. Thomas Darling Provided helpful discussions. Tumor was supplied by tbe following: Dr. Robert Ellsworth, retinoblastoma: Dr. Joseph Galicich, medulloblastoma~ Dr. Robert Touloukian, neuroblastoma. Drs. Joseph Beard and Michael A. Chirigos made available avian myeloblastosis virus. We also thank Mrs. Carol Chipkin, who gave technical assistance. Support provided by USPHS grant EYOO108-02 and USPHS grant EYO002-10.
PY - 1972/1/31
Y1 - 1972/1/31
N2 - RNA-dependent DNA-polymerase activity is present in specimens of freshly excised human medulloblastoma. This activity is demonstrated in the cell homogenates and activity remains in the supernatant fluid at 30,000g after 30 minutes and pellets at 100,000g in 2 hrs. Detergent is necessary to elicit significant activity. The enzyme activity was determined using a poly(A)·oligo(dT) template. Retinoblastoma, neuroblastoma and ocular malignant melanoma also showed activity but in much lower amounts than medulloblastoma. Normal human orbital connective tissue, cerebellum and retina, similarly studied, failed to show activity with this template. Thus, by these methods a RNA-dependent DNA-polymerase activity is demonstrated in human tumors, but not in the corresponding normal tissues.
AB - RNA-dependent DNA-polymerase activity is present in specimens of freshly excised human medulloblastoma. This activity is demonstrated in the cell homogenates and activity remains in the supernatant fluid at 30,000g after 30 minutes and pellets at 100,000g in 2 hrs. Detergent is necessary to elicit significant activity. The enzyme activity was determined using a poly(A)·oligo(dT) template. Retinoblastoma, neuroblastoma and ocular malignant melanoma also showed activity but in much lower amounts than medulloblastoma. Normal human orbital connective tissue, cerebellum and retina, similarly studied, failed to show activity with this template. Thus, by these methods a RNA-dependent DNA-polymerase activity is demonstrated in human tumors, but not in the corresponding normal tissues.
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U2 - 10.1016/S0006-291X(72)80150-5
DO - 10.1016/S0006-291X(72)80150-5
M3 - Article
C2 - 5057883
AN - SCOPUS:0015530533
SN - 0006-291X
VL - 46
SP - 383
EP - 390
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
IS - 2
ER -