Ricin does not act as an endonuclease on L cell polysomal RNA

Samuel J. Mitchell, Mary Hedblom, Daniel Cawley, L. L. Houston

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

The ability of ricin to act as an endonuclease was examined using labelled polysomes isolated from mouse L cells grown in [32P] orthophosphate. No change was detected in the electrophoretic mobility of any ribosomal RNA species whether L cells were first treated with ricin or the isolated polysomes were treated with ricin. No trichloroacetic acid soluble counts over control levels were released by ricin treatment of isolated polysomes and no changes were observed in sucrose density gradient polysome profile with ricin treatment. The action of ricin on polysomes which results in inhibition of translation does not appear to involve endonuclease activity.

Original languageEnglish (US)
Pages (from-to)763-769
Number of pages7
JournalBiochemical and Biophysical Research Communications
Volume68
Issue number3
DOIs
StatePublished - Feb 9 1976
Externally publishedYes

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Ricin
Endonucleases
Polyribosomes
RNA
Electrophoretic mobility
Trichloroacetic Acid
Ribosomal RNA
Level control
Sucrose
Phosphates
Cells

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Ricin does not act as an endonuclease on L cell polysomal RNA. / Mitchell, Samuel J.; Hedblom, Mary; Cawley, Daniel; Houston, L. L.

In: Biochemical and Biophysical Research Communications, Vol. 68, No. 3, 09.02.1976, p. 763-769.

Research output: Contribution to journalArticle

Mitchell, Samuel J. ; Hedblom, Mary ; Cawley, Daniel ; Houston, L. L. / Ricin does not act as an endonuclease on L cell polysomal RNA. In: Biochemical and Biophysical Research Communications. 1976 ; Vol. 68, No. 3. pp. 763-769.
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