RhoH is required to maintain the integrin LFA-1 in a nonadhesive state on lymphocytes

Lisa K. Cherry, Xiaoyu Li, Pascale Schwab, Bing Lim, Lloyd B. Klickstein

Research output: Contribution to journalArticle

61 Scopus citations

Abstract

Lymphocyte function-associated antigen 1 (LFA-1) is relatively nonadhesive on resting lymphocytes; however, the mechanisms underlying changes in its adhesiveness are poorly understood. In this study, we generated a Jurkat T cell clone, J+hi1.14, that contained low amounts of mRNA for RhoH, a leukocyte-specific inhibitory Rho family member. J+hi1.14 cells expressed constitutively adhesive LFA-1 and the cells bound spontaneously to intracellular adhesion molecules 1, 2 and 3. Reconstitution of RhoH mRNA expression in J+hi1.14 cells reverted the adhesion phenotype to that of wild-type. We obtained similar results using RNA interference in peripheral blood lymphocytes. These data demonstrate that RhoH is required for maintenance of lymphocyte LFA-1 in a nonadhesive state.

Original languageEnglish (US)
Pages (from-to)961-967
Number of pages7
JournalNature Immunology
Volume5
Issue number9
DOIs
StatePublished - Sep 1 2004

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

Fingerprint Dive into the research topics of 'RhoH is required to maintain the integrin LFA-1 in a nonadhesive state on lymphocytes'. Together they form a unique fingerprint.

  • Cite this