RhoH is required to maintain the integrin LFA-1 in a nonadhesive state on lymphocytes

Lisa K. Cherry, Xiaoyu Li, Pascale Schwab, Bing Lim, Lloyd B. Klickstein

Research output: Contribution to journalArticle

60 Citations (Scopus)

Abstract

Lymphocyte function-associated antigen 1 (LFA-1) is relatively nonadhesive on resting lymphocytes; however, the mechanisms underlying changes in its adhesiveness are poorly understood. In this study, we generated a Jurkat T cell clone, J+hi1.14, that contained low amounts of mRNA for RhoH, a leukocyte-specific inhibitory Rho family member. J+hi1.14 cells expressed constitutively adhesive LFA-1 and the cells bound spontaneously to intracellular adhesion molecules 1, 2 and 3. Reconstitution of RhoH mRNA expression in J+hi1.14 cells reverted the adhesion phenotype to that of wild-type. We obtained similar results using RNA interference in peripheral blood lymphocytes. These data demonstrate that RhoH is required for maintenance of lymphocyte LFA-1 in a nonadhesive state.

Original languageEnglish (US)
Pages (from-to)961-967
Number of pages7
JournalNature Immunology
Volume5
Issue number9
DOIs
StatePublished - Sep 2004

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Lymphocyte Function-Associated Antigen-1
Integrins
Lymphocytes
Adhesiveness
Messenger RNA
Jurkat Cells
RNA Interference
Cell Adhesion
Adhesives
Leukocytes
Clone Cells
Maintenance
T-Lymphocytes
Phenotype

ASJC Scopus subject areas

  • Immunology

Cite this

RhoH is required to maintain the integrin LFA-1 in a nonadhesive state on lymphocytes. / Cherry, Lisa K.; Li, Xiaoyu; Schwab, Pascale; Lim, Bing; Klickstein, Lloyd B.

In: Nature Immunology, Vol. 5, No. 9, 09.2004, p. 961-967.

Research output: Contribution to journalArticle

Cherry, Lisa K. ; Li, Xiaoyu ; Schwab, Pascale ; Lim, Bing ; Klickstein, Lloyd B. / RhoH is required to maintain the integrin LFA-1 in a nonadhesive state on lymphocytes. In: Nature Immunology. 2004 ; Vol. 5, No. 9. pp. 961-967.
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