Rhodopsin's amino terminus is a principal antigenic site

Paul A. Hargrave, Grazyna Adamus, Anatol Arendt, J. Hugh McDowell, Janet Wang, Agnieszka Szary, Donna Curtis, Robert W. Jackson

Research output: Contribution to journalArticle

45 Scopus citations

Abstract

Antisera and monoclonal antibodies to rhodopsin were examined for their binding specificity to rhodopsin by using peptides from the rhodopsin sequence as competitors for antibody binding to rhodopsin in an enzyme-linked immunoassay. Monoclonal antibodies tested were raised in mice against bovine and rat rhodopsin. Antisera tested were raised in sheep against bovine rhodopsin and in rabbits against human rhodopsin. Peptides were synthesized from the bovine rhodopsin sequences 2-32, 1-12, 13-23, 24-34, 5-11, 231-252 and 331-348 for use as competitors in the immunoassay. A mixture of soluble CNBr peptides, and the purified CNBr peptide representing the sequence 2-39 were also employed. The monoclonal antibodies were all anti-amino-terminal in their binding specificity, although each recognized slightly different regions of the amino terminus. Each of the three antisera was predominantly directed against rhodopsin's amino terminus. We conclude that the amino-terminal 30 or more amino acids, and particularly the amino-terminal 15 amino acids, represent a principal antigenic region of the rhodopsin molecule.

Original languageEnglish (US)
Pages (from-to)363-373
Number of pages11
JournalExperimental Eye Research
Volume42
Issue number4
DOIs
StatePublished - Apr 1986

Keywords

  • antigenic site
  • immunoassay
  • monoclonal antibody
  • peptide
  • rhodopsin

ASJC Scopus subject areas

  • Ophthalmology
  • Sensory Systems
  • Cellular and Molecular Neuroscience

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  • Cite this

    Hargrave, P. A., Adamus, G., Arendt, A., McDowell, J. H., Wang, J., Szary, A., Curtis, D., & Jackson, R. W. (1986). Rhodopsin's amino terminus is a principal antigenic site. Experimental Eye Research, 42(4), 363-373. https://doi.org/10.1016/0014-4835(86)90030-8