Rhesus rhadinovirus R15 encodes a functional homologue of human CD200

Carly L. Langlais, John M. Jones, Ryan D. Estep, Scott W. Wong

Research output: Contribution to journalArticle

28 Scopus citations

Abstract

A viral CD200 homologue (vCD200) encoded by open reading frame R15 of rhesus rhadinovirus (RRV), a gammaherpesvirus closely related to human herpesvirus 8 (HHV-8), is described here. RRV vCD200 shares 30% and 28% amino acid identity with human CD200 (huCD200) and HHV-8 vCD200, respectively. In vitro analysis indicated that an Fc fusion (vCD200-Fc) is expressed as a glycoprotein with a core molecular mass of 53 kDa. Utilizing monoclonal antibodies raised against vCD200-Fc, vCD200 expression was detected on the surfaces of and within supernatants from infected fibroblasts. Furthermore, in vitro assays demonstrated that vCD200-Fc treatment of monocyte-derived macrophages reduces tumor necrosis factor transcript and protein levels, implying that RRV encodes a functional vCD200.

Original languageEnglish (US)
Pages (from-to)3098-3103
Number of pages6
JournalJournal of virology
Volume80
Issue number6
DOIs
StatePublished - Mar 2006

ASJC Scopus subject areas

  • Microbiology
  • Immunology
  • Insect Science
  • Virology

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