Rhesus rhadinovirus R15 encodes a functional homologue of human CD200

Carly L. Langlais, John M. Jones, Ryan Estep, Scott Wong

Research output: Contribution to journalArticle

27 Citations (Scopus)

Abstract

A viral CD200 homologue (vCD200) encoded by open reading frame R15 of rhesus rhadinovirus (RRV), a gammaherpesvirus closely related to human herpesvirus 8 (HHV-8), is described here. RRV vCD200 shares 30% and 28% amino acid identity with human CD200 (huCD200) and HHV-8 vCD200, respectively. In vitro analysis indicated that an Fc fusion (vCD200-Fc) is expressed as a glycoprotein with a core molecular mass of 53 kDa. Utilizing monoclonal antibodies raised against vCD200-Fc, vCD200 expression was detected on the surfaces of and within supernatants from infected fibroblasts. Furthermore, in vitro assays demonstrated that vCD200-Fc treatment of monocyte-derived macrophages reduces tumor necrosis factor transcript and protein levels, implying that RRV encodes a functional vCD200.

Original languageEnglish (US)
Pages (from-to)3098-3103
Number of pages6
JournalJournal of Virology
Volume80
Issue number6
DOIs
StatePublished - Mar 2006

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Macacine herpesvirus 5
Rhadinovirus
Human herpesvirus 8
Human Herpesvirus 8
tumor necrosis factors
monocytes
Open Reading Frames
fibroblasts
open reading frames
glycoproteins
monoclonal antibodies
Glycoproteins
macrophages
Tumor Necrosis Factor-alpha
Fibroblasts
Macrophages
Monoclonal Antibodies
molecular weight
Amino Acids
amino acids

ASJC Scopus subject areas

  • Immunology

Cite this

Rhesus rhadinovirus R15 encodes a functional homologue of human CD200. / Langlais, Carly L.; Jones, John M.; Estep, Ryan; Wong, Scott.

In: Journal of Virology, Vol. 80, No. 6, 03.2006, p. 3098-3103.

Research output: Contribution to journalArticle

Langlais, Carly L. ; Jones, John M. ; Estep, Ryan ; Wong, Scott. / Rhesus rhadinovirus R15 encodes a functional homologue of human CD200. In: Journal of Virology. 2006 ; Vol. 80, No. 6. pp. 3098-3103.
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