Rhesus monkeys produced by nuclear transfer

Li Meng, John J. Ely, Richard L. Stouffer, Don P. Wolf

    Research output: Contribution to journalArticle

    165 Scopus citations

    Abstract

    Genetically identical nonhuman primates can provide a powerful animal model for gene therapy and research activities where the physiological parameters directly or indirectly under study are heritable. Here we demonstrate that nuclear transfer is a viable technology for the production of identical rhesus macaques. Oocytes recovered from gonadotropin-treated females were enucleated by aspiration of the first polar body and underlying ooplasm, then activated by cycloheximide exposure. Individual diploid blastomeres, recovered from in vitro-fertilization-produced embryos (either fresh or frozen-thawed) and used as nuclear donors, were injected under the zona pellucida of enucleated (chromosome-free) oocytes and fused by electric pulses. The reconstituted embryos were cocultured on buffalo rat liver cells before cryostorage and transfer to synchronized host mothers. Of the 9 females receiving a total of 29 reconstituted embryos, 3 became pregnant, with two live births resulting, one male and one female. The parentage of both infants was established unequivocally by genotype analysis at 7 highly variable short tandem repeat loci.

    Original languageEnglish (US)
    Pages (from-to)454-459
    Number of pages6
    JournalBiology of reproduction
    Volume57
    Issue number2
    DOIs
    StatePublished - Aug 27 1997

    ASJC Scopus subject areas

    • Reproductive Medicine
    • Cell Biology

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