Rhesus monkey embryos produced by nuclear transfer from embryonic blastomeres or somatic cells

Shoukhrat Mitalipov, Richard R. Yeoman, Kevin D. Nusser, Don P. Wolf

    Research output: Contribution to journalArticle

    65 Citations (Scopus)

    Abstract

    Production of genetically identical nonhuman primates would reduce the number of animals required for biomedical research and dramatically impact studies pertaining to immune system function, such as development of the human-immuno-deficiency-virus vaccine. Our long-term goal is to develop robust somatic cell cloning and/or twinning protocols in the rhesus macaque. The objective of this study was to determine the developmental competence of nuclear transfer (NT) embryos derived from embryonic blastomeres (embryonic cell NT) or fetal fibroblasts (somatic cell NT) as a first step in the production of rhesus monkeys by somatic cell cloning. Development of cleaved embryos up to the 8-cell stage was similar among embryonic and somatic cell NT embryos and comparable to controls created by intracytoplasmic sperm injection (ICSI; mean ± SEM, 81 ± 5%, 88 ± 7%, and 87 ± 4%, respectively). However, significantly lower rates of development to the blastocyst stage were observed with somatic cell NT embryos (1%) in contrast to embryonic cell NT (34 ± 15%) or ICSI control embryos (46 ± 6%). Development of somatic cell NT embryos was not markedly affected by donor cell treatment, timing of activation, or chemical activation protocol. Transfer of embryonic, but not of somatic cell NT embryos, into recipients resulted in term pregnancy. Future efforts will focus on optimizing the production of somatic cell NT embryos that develop in high efficiency to the blastocyst stage in vitro.

    Original languageEnglish (US)
    Pages (from-to)1367-1373
    Number of pages7
    JournalBiology of Reproduction
    Volume66
    Issue number5
    StatePublished - 2002

    Fingerprint

    Blastomeres
    Macaca mulatta
    Embryonic Structures
    Embryo Transfer
    Intracytoplasmic Sperm Injections
    Blastocyst
    Organism Cloning
    Human Development
    Mental Competency
    Primates
    Embryonic Development
    Biomedical Research
    Immune System
    Vaccines
    Fibroblasts

    Keywords

    • Assisted reproductive technology
    • Developmental biology
    • Early development
    • Embryo
    • Fertilization
    • Implantation

    ASJC Scopus subject areas

    • Cell Biology
    • Developmental Biology
    • Embryology

    Cite this

    Rhesus monkey embryos produced by nuclear transfer from embryonic blastomeres or somatic cells. / Mitalipov, Shoukhrat; Yeoman, Richard R.; Nusser, Kevin D.; Wolf, Don P.

    In: Biology of Reproduction, Vol. 66, No. 5, 2002, p. 1367-1373.

    Research output: Contribution to journalArticle

    Mitalipov, Shoukhrat ; Yeoman, Richard R. ; Nusser, Kevin D. ; Wolf, Don P. / Rhesus monkey embryos produced by nuclear transfer from embryonic blastomeres or somatic cells. In: Biology of Reproduction. 2002 ; Vol. 66, No. 5. pp. 1367-1373.
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