RHEB1 expression in embryonic and postnatal mouse

Qi Tian, James L. Smart, Joachim H. Clement, Yingming Wang, Alex Derkatch, Harald Schubert, Michael Danilchik, Daniel Marks, Lev Fedorov

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Ras homolog enriched in brain (RHEB1) is a member within the superfamily of GTP-binding proteins encoded by the RAS oncogenes. RHEB1 is located at the crossroad of several important pathways including the insulin-signaling pathways and thus plays an important role in different physiological processes. To understand better the physiological relevance of RHEB1 protein, the expression pattern of RHEB1 was analyzed in both embryonic (at E3.5–E16.5) and adult (1-month old) mice. RHEB1 immunostaining and X-gal staining were used for wild-type and Rheb1 gene trap mutant mice, respectively. These independent methods revealed similar RHEB1 expression patterns during both embryonic and postnatal developments. Ubiquitous uniform RHEB1/β-gal and/or RHEB1 expression was seen in preimplantation embryos at E3.5 and postimplantation embryos up to E12.5. Between stages E13.5 and E16.5, RHEB1 expression levels became complex: In particular, strong expression was identified in neural tissues, including the neuroepithelial layer of the mesencephalon, telencephalon, and neural tube of CNS and dorsal root ganglia. In addition, strong expression was seen in certain peripheral tissues including heart, intestine, muscle, and urinary bladder. Postnatal mice have broad spatial RHEB1 expression in different regions of the cerebral cortex, subcortical regions (including hippocampus), olfactory bulb, medulla oblongata, and cerebellum (particularly in Purkinje cells). Significant RHEB1 expression was also viewed in internal organs including the heart, intestine, urinary bladder, and muscle. Moreover, adult animals have complex tissue- and organ-specific RHEB1 expression patterns with different intensities observed throughout postnatal development. Its expression level is in general comparable in CNS and other organs of mouse. Thus, the expression pattern of RHEB1 suggests that it likely plays a ubiquitous role in the development of the early embryo with more tissue-specific roles in later development.

Original languageEnglish (US)
Pages (from-to)1-12
Number of pages12
JournalHistochemistry and Cell Biology
DOIs
StateAccepted/In press - Dec 26 2015

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Intestines
Embryonic Development
Urinary Bladder
Physiological Phenomena
Telencephalon
Medulla Oblongata
Neural Tube
Olfactory Bulb
Purkinje Cells
Spinal Ganglia
Blastocyst
Mesencephalon
Oncogenes
GTP-Binding Proteins
Cerebral Cortex
Cerebellum
Hippocampus
Myocardium
Embryonic Structures
Insulin

Keywords

  • Adult mice
  • Embryos
  • Expression pattern
  • Gene trap
  • Immunohistochemistry
  • Rheb1 gene
  • X-gal staining

ASJC Scopus subject areas

  • Cell Biology
  • Histology
  • Medical Laboratory Technology
  • Molecular Biology

Cite this

Tian, Q., Smart, J. L., Clement, J. H., Wang, Y., Derkatch, A., Schubert, H., ... Fedorov, L. (Accepted/In press). RHEB1 expression in embryonic and postnatal mouse. Histochemistry and Cell Biology, 1-12. https://doi.org/10.1007/s00418-015-1394-3

RHEB1 expression in embryonic and postnatal mouse. / Tian, Qi; Smart, James L.; Clement, Joachim H.; Wang, Yingming; Derkatch, Alex; Schubert, Harald; Danilchik, Michael; Marks, Daniel; Fedorov, Lev.

In: Histochemistry and Cell Biology, 26.12.2015, p. 1-12.

Research output: Contribution to journalArticle

Tian Q, Smart JL, Clement JH, Wang Y, Derkatch A, Schubert H et al. RHEB1 expression in embryonic and postnatal mouse. Histochemistry and Cell Biology. 2015 Dec 26;1-12. https://doi.org/10.1007/s00418-015-1394-3
Tian, Qi ; Smart, James L. ; Clement, Joachim H. ; Wang, Yingming ; Derkatch, Alex ; Schubert, Harald ; Danilchik, Michael ; Marks, Daniel ; Fedorov, Lev. / RHEB1 expression in embryonic and postnatal mouse. In: Histochemistry and Cell Biology. 2015 ; pp. 1-12.
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