Reversible acetaldehyde inhibition of A23187-stimulated amylase secretion from isolated rat pancreatic acini

M. B. Lewin, A. Wong, Clifford Deveney, H. Sankaran

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

The Ca2+ ionophore, A23187, stimulated amylase secretion from isolated rat pancreatic acini in a dosedependent manner with a maximal effect at 6 μM. Acetaldehyde, a metabolite of ethanol, caused a reduction in the magnitude of ionophore-stimulated secretion with no evidence of competitive inhibition. Furthermore, 6 μM ionophore-stimulated amylase secretion was dose-dependently inhibited by acetaldehyde. This inhibitory effect of acetaldehyde, however, was reversible on washing and reincubating acetaldehyde-treated acini. These results suggest that acetaldehyde reversibly inhibits intracellular components mediating stimulated secretion and this inhibition requires a continuous chemical interaction between acetaldehyde and intracellular component(s) regulating stimulated enzyme secretion.

Original languageEnglish (US)
Pages (from-to)259-262
Number of pages4
JournalFEBS Letters
Volume184
Issue number2
DOIs
StatePublished - May 20 1985
Externally publishedYes

Fingerprint

Acetaldehyde
Calcimycin
Amylases
Rats
Ionophores
Metabolites
Washing
Ethanol
Enzymes

Keywords

  • Acetaldehyde Pancreatic acini Calcium ionophore Amylase secretion Reversibility

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Reversible acetaldehyde inhibition of A23187-stimulated amylase secretion from isolated rat pancreatic acini. / Lewin, M. B.; Wong, A.; Deveney, Clifford; Sankaran, H.

In: FEBS Letters, Vol. 184, No. 2, 20.05.1985, p. 259-262.

Research output: Contribution to journalArticle

@article{17e2138509a34c4286336dbbeeda5006,
title = "Reversible acetaldehyde inhibition of A23187-stimulated amylase secretion from isolated rat pancreatic acini",
abstract = "The Ca2+ ionophore, A23187, stimulated amylase secretion from isolated rat pancreatic acini in a dosedependent manner with a maximal effect at 6 μM. Acetaldehyde, a metabolite of ethanol, caused a reduction in the magnitude of ionophore-stimulated secretion with no evidence of competitive inhibition. Furthermore, 6 μM ionophore-stimulated amylase secretion was dose-dependently inhibited by acetaldehyde. This inhibitory effect of acetaldehyde, however, was reversible on washing and reincubating acetaldehyde-treated acini. These results suggest that acetaldehyde reversibly inhibits intracellular components mediating stimulated secretion and this inhibition requires a continuous chemical interaction between acetaldehyde and intracellular component(s) regulating stimulated enzyme secretion.",
keywords = "Acetaldehyde Pancreatic acini Calcium ionophore Amylase secretion Reversibility",
author = "Lewin, {M. B.} and A. Wong and Clifford Deveney and H. Sankaran",
year = "1985",
month = "5",
day = "20",
doi = "10.1016/0014-5793(85)80618-9",
language = "English (US)",
volume = "184",
pages = "259--262",
journal = "FEBS Letters",
issn = "0014-5793",
publisher = "Elsevier",
number = "2",

}

TY - JOUR

T1 - Reversible acetaldehyde inhibition of A23187-stimulated amylase secretion from isolated rat pancreatic acini

AU - Lewin, M. B.

AU - Wong, A.

AU - Deveney, Clifford

AU - Sankaran, H.

PY - 1985/5/20

Y1 - 1985/5/20

N2 - The Ca2+ ionophore, A23187, stimulated amylase secretion from isolated rat pancreatic acini in a dosedependent manner with a maximal effect at 6 μM. Acetaldehyde, a metabolite of ethanol, caused a reduction in the magnitude of ionophore-stimulated secretion with no evidence of competitive inhibition. Furthermore, 6 μM ionophore-stimulated amylase secretion was dose-dependently inhibited by acetaldehyde. This inhibitory effect of acetaldehyde, however, was reversible on washing and reincubating acetaldehyde-treated acini. These results suggest that acetaldehyde reversibly inhibits intracellular components mediating stimulated secretion and this inhibition requires a continuous chemical interaction between acetaldehyde and intracellular component(s) regulating stimulated enzyme secretion.

AB - The Ca2+ ionophore, A23187, stimulated amylase secretion from isolated rat pancreatic acini in a dosedependent manner with a maximal effect at 6 μM. Acetaldehyde, a metabolite of ethanol, caused a reduction in the magnitude of ionophore-stimulated secretion with no evidence of competitive inhibition. Furthermore, 6 μM ionophore-stimulated amylase secretion was dose-dependently inhibited by acetaldehyde. This inhibitory effect of acetaldehyde, however, was reversible on washing and reincubating acetaldehyde-treated acini. These results suggest that acetaldehyde reversibly inhibits intracellular components mediating stimulated secretion and this inhibition requires a continuous chemical interaction between acetaldehyde and intracellular component(s) regulating stimulated enzyme secretion.

KW - Acetaldehyde Pancreatic acini Calcium ionophore Amylase secretion Reversibility

UR - http://www.scopus.com/inward/record.url?scp=0021889095&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0021889095&partnerID=8YFLogxK

U2 - 10.1016/0014-5793(85)80618-9

DO - 10.1016/0014-5793(85)80618-9

M3 - Article

C2 - 2581809

AN - SCOPUS:0021889095

VL - 184

SP - 259

EP - 262

JO - FEBS Letters

JF - FEBS Letters

SN - 0014-5793

IS - 2

ER -