Benzoate and naphthoate esters of C5 to C20 isoprenoid alcohols are excellent derivatives for reversed-phase liquid chromatography. Nonlinear responses of log k' to changes in the composition of the mobile phase are observed on Radial PAK C18 columns. In general, the capacity factors (k') increased with increases in carbon content and decreased with increases in the number of double bonds, branching, and Z double bond content. The large extinction coefficients of the aromatic ester moieties, especially the 2-naphthoates, provide high sensitivity for UV detection.
|Original language||English (US)|
|Number of pages||12|
|Journal||Methods in Enzymology|
|State||Published - Jan 1 1985|
ASJC Scopus subject areas
- Molecular Biology