Reversed-phase high-performance liquid chromatography of C5 to C20 isoprenoid benzoates and naphthoates

T. H. Yang, T. M. Zabriskie, C. D. Poulter

Research output: Contribution to journalArticle

3 Scopus citations

Abstract

Benzoate and naphthoate esters of C5 to C20 isoprenoid alcohols are excellent derivatives for reversed-phase liquid chromatography. Nonlinear responses of log k' to changes in the composition of the mobile phase are observed on Radial PAK C18 columns. In general, the capacity factors (k') increased with increases in carbon content and decreased with increases in the number of double bonds, branching, and Z double bond content. The large extinction coefficients of the aromatic ester moieties, especially the 2-naphthoates, provide high sensitivity for UV detection.

Original languageEnglish (US)
Pages (from-to)252-263
Number of pages12
JournalMethods in Enzymology
VolumeVOL. 111
StatePublished - Jan 1 1985

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology

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