Reverse Transcription Polymerase Chain Reaction Analysis of Pituitary Hormone, Pit-1 and Steroidogenic Factor-1 Messenger RNA Expression in Pituitary Tumors

Michael T. McDermott, Bryan R. Haugen, David F. Gordon, William M. Wood, Nicole S. Brown, Carol A. Bauer, Maureen J. Garrity, Bette K. Kleinschmidt-DeMasters, Kevin O. Lillehei, Mary Samuels, Tamis M. Bright, E. Chester Ridgway

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Pit-1 is a transcription factor that appears early in embryonic pituitary gland formation and is necessary for the development of somatotropes, lactotropes and thyrotropes. Steroidogenic factor-1 (SF-1) is another early appearing transcription factor that is involved in the development of gonadotropes. In this study we have compared RT-PCR analysis of hormone mRNA with traditional IHC for classification of 27 pituitary tumors and have evaluated the correlation of Pit-1 and SF-1 mRNA with hormone mRNA. RT-PCR detected concordant hormone mRNA in 100% of GH IHC positive, 100% of PRL IHC positive, 33% of TSH IHC positive, and 93% of gonadotropin IHC positive tumors. IHC, however, was concordant in only 71% of GH mRNA positive, 78% of PRL mRNA positive, 17% of TSHβ mRNA positive, and 76% of FSHβ mRNA positive tumors. Pit-1 mRNA was positive in 87% of tumors in which mRNA for GH, PRL or TSHβ was detected and in only 17% of GH, PRL and TSHβ mRNA negative tumors. SF-1 mRNA was positive in 94% of tumors in which mRNA for FSHβ was present and in no FSHβ mRNA negative tumors. We conclude that RT-PCR analysis of hormone mRNA may be more sensitive than traditional hormone IHC for classification of pituitary tumors. Furthermore, tumor Pit-1 mRNA positively correlates with GH, PRL and TSHβ mRNA while tumor SF-1 mRNA correlates well with FSHβ mRNA. Combined analysis of hormone and transcription factor mRNA in pituitary tumor tissue may therefore be a more meaningful approach to pituitary tumor characterization.

Original languageEnglish (US)
Pages (from-to)217-224
Number of pages8
JournalPituitary
Volume2
Issue number3
StatePublished - 1999
Externally publishedYes

Fingerprint

Steroidogenic Factor 1
Pituitary Hormones
Pituitary Neoplasms
Reverse Transcription
Polymerase Chain Reaction
Messenger RNA
Hormones
Neoplasms
Transcription Factors

Keywords

  • Pit-1
  • Pituitary tumors
  • Steroidogenic factor-1

ASJC Scopus subject areas

  • Endocrinology

Cite this

McDermott, M. T., Haugen, B. R., Gordon, D. F., Wood, W. M., Brown, N. S., Bauer, C. A., ... Ridgway, E. C. (1999). Reverse Transcription Polymerase Chain Reaction Analysis of Pituitary Hormone, Pit-1 and Steroidogenic Factor-1 Messenger RNA Expression in Pituitary Tumors. Pituitary, 2(3), 217-224.

Reverse Transcription Polymerase Chain Reaction Analysis of Pituitary Hormone, Pit-1 and Steroidogenic Factor-1 Messenger RNA Expression in Pituitary Tumors. / McDermott, Michael T.; Haugen, Bryan R.; Gordon, David F.; Wood, William M.; Brown, Nicole S.; Bauer, Carol A.; Garrity, Maureen J.; Kleinschmidt-DeMasters, Bette K.; Lillehei, Kevin O.; Samuels, Mary; Bright, Tamis M.; Ridgway, E. Chester.

In: Pituitary, Vol. 2, No. 3, 1999, p. 217-224.

Research output: Contribution to journalArticle

McDermott, MT, Haugen, BR, Gordon, DF, Wood, WM, Brown, NS, Bauer, CA, Garrity, MJ, Kleinschmidt-DeMasters, BK, Lillehei, KO, Samuels, M, Bright, TM & Ridgway, EC 1999, 'Reverse Transcription Polymerase Chain Reaction Analysis of Pituitary Hormone, Pit-1 and Steroidogenic Factor-1 Messenger RNA Expression in Pituitary Tumors', Pituitary, vol. 2, no. 3, pp. 217-224.
McDermott, Michael T. ; Haugen, Bryan R. ; Gordon, David F. ; Wood, William M. ; Brown, Nicole S. ; Bauer, Carol A. ; Garrity, Maureen J. ; Kleinschmidt-DeMasters, Bette K. ; Lillehei, Kevin O. ; Samuels, Mary ; Bright, Tamis M. ; Ridgway, E. Chester. / Reverse Transcription Polymerase Chain Reaction Analysis of Pituitary Hormone, Pit-1 and Steroidogenic Factor-1 Messenger RNA Expression in Pituitary Tumors. In: Pituitary. 1999 ; Vol. 2, No. 3. pp. 217-224.
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abstract = "Pit-1 is a transcription factor that appears early in embryonic pituitary gland formation and is necessary for the development of somatotropes, lactotropes and thyrotropes. Steroidogenic factor-1 (SF-1) is another early appearing transcription factor that is involved in the development of gonadotropes. In this study we have compared RT-PCR analysis of hormone mRNA with traditional IHC for classification of 27 pituitary tumors and have evaluated the correlation of Pit-1 and SF-1 mRNA with hormone mRNA. RT-PCR detected concordant hormone mRNA in 100{\%} of GH IHC positive, 100{\%} of PRL IHC positive, 33{\%} of TSH IHC positive, and 93{\%} of gonadotropin IHC positive tumors. IHC, however, was concordant in only 71{\%} of GH mRNA positive, 78{\%} of PRL mRNA positive, 17{\%} of TSHβ mRNA positive, and 76{\%} of FSHβ mRNA positive tumors. Pit-1 mRNA was positive in 87{\%} of tumors in which mRNA for GH, PRL or TSHβ was detected and in only 17{\%} of GH, PRL and TSHβ mRNA negative tumors. SF-1 mRNA was positive in 94{\%} of tumors in which mRNA for FSHβ was present and in no FSHβ mRNA negative tumors. We conclude that RT-PCR analysis of hormone mRNA may be more sensitive than traditional hormone IHC for classification of pituitary tumors. Furthermore, tumor Pit-1 mRNA positively correlates with GH, PRL and TSHβ mRNA while tumor SF-1 mRNA correlates well with FSHβ mRNA. Combined analysis of hormone and transcription factor mRNA in pituitary tumor tissue may therefore be a more meaningful approach to pituitary tumor characterization.",
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AU - Haugen, Bryan R.

AU - Gordon, David F.

AU - Wood, William M.

AU - Brown, Nicole S.

AU - Bauer, Carol A.

AU - Garrity, Maureen J.

AU - Kleinschmidt-DeMasters, Bette K.

AU - Lillehei, Kevin O.

AU - Samuels, Mary

AU - Bright, Tamis M.

AU - Ridgway, E. Chester

PY - 1999

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N2 - Pit-1 is a transcription factor that appears early in embryonic pituitary gland formation and is necessary for the development of somatotropes, lactotropes and thyrotropes. Steroidogenic factor-1 (SF-1) is another early appearing transcription factor that is involved in the development of gonadotropes. In this study we have compared RT-PCR analysis of hormone mRNA with traditional IHC for classification of 27 pituitary tumors and have evaluated the correlation of Pit-1 and SF-1 mRNA with hormone mRNA. RT-PCR detected concordant hormone mRNA in 100% of GH IHC positive, 100% of PRL IHC positive, 33% of TSH IHC positive, and 93% of gonadotropin IHC positive tumors. IHC, however, was concordant in only 71% of GH mRNA positive, 78% of PRL mRNA positive, 17% of TSHβ mRNA positive, and 76% of FSHβ mRNA positive tumors. Pit-1 mRNA was positive in 87% of tumors in which mRNA for GH, PRL or TSHβ was detected and in only 17% of GH, PRL and TSHβ mRNA negative tumors. SF-1 mRNA was positive in 94% of tumors in which mRNA for FSHβ was present and in no FSHβ mRNA negative tumors. We conclude that RT-PCR analysis of hormone mRNA may be more sensitive than traditional hormone IHC for classification of pituitary tumors. Furthermore, tumor Pit-1 mRNA positively correlates with GH, PRL and TSHβ mRNA while tumor SF-1 mRNA correlates well with FSHβ mRNA. Combined analysis of hormone and transcription factor mRNA in pituitary tumor tissue may therefore be a more meaningful approach to pituitary tumor characterization.

AB - Pit-1 is a transcription factor that appears early in embryonic pituitary gland formation and is necessary for the development of somatotropes, lactotropes and thyrotropes. Steroidogenic factor-1 (SF-1) is another early appearing transcription factor that is involved in the development of gonadotropes. In this study we have compared RT-PCR analysis of hormone mRNA with traditional IHC for classification of 27 pituitary tumors and have evaluated the correlation of Pit-1 and SF-1 mRNA with hormone mRNA. RT-PCR detected concordant hormone mRNA in 100% of GH IHC positive, 100% of PRL IHC positive, 33% of TSH IHC positive, and 93% of gonadotropin IHC positive tumors. IHC, however, was concordant in only 71% of GH mRNA positive, 78% of PRL mRNA positive, 17% of TSHβ mRNA positive, and 76% of FSHβ mRNA positive tumors. Pit-1 mRNA was positive in 87% of tumors in which mRNA for GH, PRL or TSHβ was detected and in only 17% of GH, PRL and TSHβ mRNA negative tumors. SF-1 mRNA was positive in 94% of tumors in which mRNA for FSHβ was present and in no FSHβ mRNA negative tumors. We conclude that RT-PCR analysis of hormone mRNA may be more sensitive than traditional hormone IHC for classification of pituitary tumors. Furthermore, tumor Pit-1 mRNA positively correlates with GH, PRL and TSHβ mRNA while tumor SF-1 mRNA correlates well with FSHβ mRNA. Combined analysis of hormone and transcription factor mRNA in pituitary tumor tissue may therefore be a more meaningful approach to pituitary tumor characterization.

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