Reverse Transcription Polymerase Chain Reaction Analysis of Pituitary Hormone, Pit-1 and Steroidogenic Factor-1 Messenger RNA Expression in Pituitary Tumors

Michael T. McDermott, Bryan R. Haugen, David F. Gordon, William M. Wood, Nicole S. Brown, Carol A. Bauer, Maureen J. Garrity, Bette K. Kleinschmidt-DeMasters, Kevin O. Lillehei, Mary H. Samuels, Tamis M. Bright, E. Chester Ridgway

Research output: Contribution to journalArticle

1 Scopus citations

Abstract

Pit-1 is a transcription factor that appears early in embryonic pituitary gland formation and is necessary for the development of somatotropes, lactotropes and thyrotropes. Steroidogenic factor-1 (SF-1) is another early appearing transcription factor that is involved in the development of gonadotropes. In this study we have compared RT-PCR analysis of hormone mRNA with traditional IHC for classification of 27 pituitary tumors and have evaluated the correlation of Pit-1 and SF-1 mRNA with hormone mRNA. RT-PCR detected concordant hormone mRNA in 100% of GH IHC positive, 100% of PRL IHC positive, 33% of TSH IHC positive, and 93% of gonadotropin IHC positive tumors. IHC, however, was concordant in only 71% of GH mRNA positive, 78% of PRL mRNA positive, 17% of TSHβ mRNA positive, and 76% of FSHβ mRNA positive tumors. Pit-1 mRNA was positive in 87% of tumors in which mRNA for GH, PRL or TSHβ was detected and in only 17% of GH, PRL and TSHβ mRNA negative tumors. SF-1 mRNA was positive in 94% of tumors in which mRNA for FSHβ was present and in no FSHβ mRNA negative tumors. We conclude that RT-PCR analysis of hormone mRNA may be more sensitive than traditional hormone IHC for classification of pituitary tumors. Furthermore, tumor Pit-1 mRNA positively correlates with GH, PRL and TSHβ mRNA while tumor SF-1 mRNA correlates well with FSHβ mRNA. Combined analysis of hormone and transcription factor mRNA in pituitary tumor tissue may therefore be a more meaningful approach to pituitary tumor characterization.

Original languageEnglish (US)
Pages (from-to)217-224
Number of pages8
JournalPituitary
Volume2
Issue number3
DOIs
StatePublished - Jan 1 1999

Keywords

  • Pit-1
  • Pituitary tumors
  • Steroidogenic factor-1

ASJC Scopus subject areas

  • Endocrinology, Diabetes and Metabolism
  • Endocrinology

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    McDermott, M. T., Haugen, B. R., Gordon, D. F., Wood, W. M., Brown, N. S., Bauer, C. A., Garrity, M. J., Kleinschmidt-DeMasters, B. K., Lillehei, K. O., Samuels, M. H., Bright, T. M., & Ridgway, E. C. (1999). Reverse Transcription Polymerase Chain Reaction Analysis of Pituitary Hormone, Pit-1 and Steroidogenic Factor-1 Messenger RNA Expression in Pituitary Tumors. Pituitary, 2(3), 217-224. https://doi.org/10.1023/A:1009957411973