Reverse phase protein array identifies novel anti-invasion mechanisms of YC-1

Bo Hong, Vivian W.Y. Lui, Edwin P. Hui, Yiling Lu, Horasis S.Y. Leung, Elaine Y.L. Wong, Suk Hang Cheng, Margaret H.L. Ng, Gordon Mills, Anthony T.C. Chan

Research output: Contribution to journalArticle

16 Scopus citations


YC-1 has recently been demonstrated to have potent anti-invasion and anti-metastatic activity in several cancer models, in addition to its anti-proliferation activity. However, the mechanism underlying its anti-invasion/anti-metastatic activity is largely unknown. Nasopharyngeal carcinoma (NPC) is a highly metastatic head and neck cancer in Southeast Asia. Here, we demonstrated that YC-1 inhibited invasiveness and proliferation of NPC cells, with the latter being accompanied by PARP cleavage, S-phase arrest and activation of Chk1/Chk2. We aimed at identifying novel anti-invasion mechanisms of YC-1 in NPC by a functional proteomic platform, the reverse phase protein array (RPPA). Our study revealed for the first time that multiple invasion-related signaling proteins (β-catenin, caveolin, Src and EGFR), as well as several growth-related proteins (AMPKα, phospho-acetyl-CoA carboxylase (p-ACC), HER-2 and mTOR), which were previously un-described signaling proteins altered by YC-1, were found to be down-modulated by YC-1 in NPC cells. We hypothesized that YC-1-mediated downregulation of these invasion proteins contributed to its anti-invasion activity in NPC cells. Overexpression of EGFR, activated Src or caveolin, but not β-catenin reversed the inhibitory effects of YC-1 on NPC cell invasion, with EGFR and activated Src having additional effects on rescuing NPC cells from YC-1-mediated growth inhibition. In summary, we have identified several novel anti-invasion mechanisms of YC-1 that could impact NPC, and possibly other cancers as well.

Original languageEnglish (US)
Pages (from-to)842-852
Number of pages11
JournalBiochemical Pharmacology
Issue number6
Publication statusPublished - Mar 15 2010
Externally publishedYes


  • Anti-invasion
  • Anti-proliferation
  • Nasopharyngeal carcinoma (NPC)
  • Reverse phase protein array
  • YC-1 mechanisms

ASJC Scopus subject areas

  • Biochemistry
  • Pharmacology

Cite this

Hong, B., Lui, V. W. Y., Hui, E. P., Lu, Y., Leung, H. S. Y., Wong, E. Y. L., ... Chan, A. T. C. (2010). Reverse phase protein array identifies novel anti-invasion mechanisms of YC-1. Biochemical Pharmacology, 79(6), 842-852.