Retrieval of human cytomegalovirus glycoprotein B from cell surface is not required for virus envelopment in astrocytoma cells

Michael A. Jarvis, Kenneth N. Fish, Cecilia Söderberg-Naucler, Daniel Streblow, Heather L. Meyers, Gary Thomas, Jay Nelson

Research output: Contribution to journalArticle

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Abstract

Human cytomegalovirus (HCMV) is a prototypic member of the betaherpesvirus family. The HCMV virion is composed of a large DNA genome encapsidated within a nucleocapsid, which is wrapped within an inner proteinaceous tegument and an outer lipid envelope containing viral glycoproteins. Although genome encapsidation clearly occurs in the nucleus, the subsequent steps in the virion assembly process are unclear. HCMV glycoprotein B (gB) is a major component of the virion envelope that plays a critical role in virus entry and is essential for the production of infectious virus progeny. The aim of our present study was to identify the secretory compartment to which HCMV gB was localized and to investigate the role of endocytosis in mediating gB localization and HCMV biogenesis. We show that HCMV gB is localized to the trans-Golgi network (TGN) in HCMV-infected cells and that gB contains all of the trafficking information necessary for TGN localization. Endocytosis of gB was shown to play a role in mediating TGN localization of gB and in targeting of the protein to the site of virus envelopment. However, inhibition of endocytosis with a dominant-negative dynamin I molecule did not affect the production of infectious virus. These observations indicate that, although endocytosis is involved in the trafficking of gB to the site of glycoprotein accumulation in the TGN, endocytosis of gB is not required for the production of infectious HCMV.

Original languageEnglish (US)
Pages (from-to)5147-5155
Number of pages9
JournalJournal of Virology
Volume76
Issue number10
DOIs
StatePublished - 2002

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Human herpesvirus 5
Astrocytoma
glycoproteins
Glycoproteins
B-Lymphocytes
Viruses
trans-Golgi Network
Endocytosis
viruses
Cytomegalovirus
endocytosis
Virion
cells
virion
Dynamin I
Genome
Nucleocapsid
Virus Internalization
Simplexvirus glycoprotein B
Protein Transport

ASJC Scopus subject areas

  • Immunology

Cite this

Retrieval of human cytomegalovirus glycoprotein B from cell surface is not required for virus envelopment in astrocytoma cells. / Jarvis, Michael A.; Fish, Kenneth N.; Söderberg-Naucler, Cecilia; Streblow, Daniel; Meyers, Heather L.; Thomas, Gary; Nelson, Jay.

In: Journal of Virology, Vol. 76, No. 10, 2002, p. 5147-5155.

Research output: Contribution to journalArticle

Jarvis, Michael A. ; Fish, Kenneth N. ; Söderberg-Naucler, Cecilia ; Streblow, Daniel ; Meyers, Heather L. ; Thomas, Gary ; Nelson, Jay. / Retrieval of human cytomegalovirus glycoprotein B from cell surface is not required for virus envelopment in astrocytoma cells. In: Journal of Virology. 2002 ; Vol. 76, No. 10. pp. 5147-5155.
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