Retinoic acid signaling is necessary for the development of the organ of Corti

Yael Raz, Matthew W. Kelley

Research output: Contribution to journalArticle

44 Citations (Scopus)

Abstract

The cellular mosaic of the mammalian organ of Corti represents one of the most highly ordered structures in any vertebrate system. A single row of inner hair cells and three or four rows of outer hair cells extend along the basal-to-apical axis of the cochlea. The factors that play a role in the development of specific cell types within the cochlea are largely unknown; however, the results of previous studies have strongly suggested that retinoic acid plays a role in the development of cells as hair cells. To determine whether cochlear progenitor cells can respond directly to retinoic acid, the expression patterns for each of the RAR and RXR receptors within the embryonic cochlear duct were determined by in situ hybridization. Results indicate that RARα, RXRα, and RXRγ are initially expressed throughout the cochlear duct. As development continues, the expression of each receptor becomes more intense in cells that will develop as hair cells. At the same time, receptor expression is down-regulated in cells that will develop as nonsensory cell types. To determine the effects of retinoic acid signaling during the development of the organ of Corti, activation of retinoid receptors was blocked in cultures of the embryonic cochlea through receptor-specific antagonism or inhibition of retinoic acid synthesis. Results indicate that inhibition of retinoic acid signaling induces a significant decrease in the number of cells that develop as hair cells and a disruption in the development of the organ of Corti. These results demonstrate that cells within the developing cochlea can respond to retinoic acid and that signaling by retinoic acid is necessary for the normal development of the organ of Corti.

Original languageEnglish (US)
Pages (from-to)180-193
Number of pages14
JournalDevelopmental Biology
Volume213
Issue number1
DOIs
StatePublished - Sep 1 1999
Externally publishedYes

Fingerprint

Organ of Corti
Tretinoin
Cochlea
Cochlear Duct
Outer Auditory Hair Cells
Inner Auditory Hair Cells
Retinoids
In Situ Hybridization
Vertebrates
Stem Cells
Cell Count

Keywords

  • Cochlea
  • Differentiation
  • Hair cells
  • Hearing
  • Retinoids

ASJC Scopus subject areas

  • Developmental Biology

Cite this

Retinoic acid signaling is necessary for the development of the organ of Corti. / Raz, Yael; Kelley, Matthew W.

In: Developmental Biology, Vol. 213, No. 1, 01.09.1999, p. 180-193.

Research output: Contribution to journalArticle

@article{86760456f04e4cb098c53285014ec1eb,
title = "Retinoic acid signaling is necessary for the development of the organ of Corti",
abstract = "The cellular mosaic of the mammalian organ of Corti represents one of the most highly ordered structures in any vertebrate system. A single row of inner hair cells and three or four rows of outer hair cells extend along the basal-to-apical axis of the cochlea. The factors that play a role in the development of specific cell types within the cochlea are largely unknown; however, the results of previous studies have strongly suggested that retinoic acid plays a role in the development of cells as hair cells. To determine whether cochlear progenitor cells can respond directly to retinoic acid, the expression patterns for each of the RAR and RXR receptors within the embryonic cochlear duct were determined by in situ hybridization. Results indicate that RARα, RXRα, and RXRγ are initially expressed throughout the cochlear duct. As development continues, the expression of each receptor becomes more intense in cells that will develop as hair cells. At the same time, receptor expression is down-regulated in cells that will develop as nonsensory cell types. To determine the effects of retinoic acid signaling during the development of the organ of Corti, activation of retinoid receptors was blocked in cultures of the embryonic cochlea through receptor-specific antagonism or inhibition of retinoic acid synthesis. Results indicate that inhibition of retinoic acid signaling induces a significant decrease in the number of cells that develop as hair cells and a disruption in the development of the organ of Corti. These results demonstrate that cells within the developing cochlea can respond to retinoic acid and that signaling by retinoic acid is necessary for the normal development of the organ of Corti.",
keywords = "Cochlea, Differentiation, Hair cells, Hearing, Retinoids",
author = "Yael Raz and Kelley, {Matthew W.}",
year = "1999",
month = "9",
day = "1",
doi = "10.1006/dbio.1999.9364",
language = "English (US)",
volume = "213",
pages = "180--193",
journal = "Developmental Biology",
issn = "0012-1606",
publisher = "Academic Press Inc.",
number = "1",

}

TY - JOUR

T1 - Retinoic acid signaling is necessary for the development of the organ of Corti

AU - Raz, Yael

AU - Kelley, Matthew W.

PY - 1999/9/1

Y1 - 1999/9/1

N2 - The cellular mosaic of the mammalian organ of Corti represents one of the most highly ordered structures in any vertebrate system. A single row of inner hair cells and three or four rows of outer hair cells extend along the basal-to-apical axis of the cochlea. The factors that play a role in the development of specific cell types within the cochlea are largely unknown; however, the results of previous studies have strongly suggested that retinoic acid plays a role in the development of cells as hair cells. To determine whether cochlear progenitor cells can respond directly to retinoic acid, the expression patterns for each of the RAR and RXR receptors within the embryonic cochlear duct were determined by in situ hybridization. Results indicate that RARα, RXRα, and RXRγ are initially expressed throughout the cochlear duct. As development continues, the expression of each receptor becomes more intense in cells that will develop as hair cells. At the same time, receptor expression is down-regulated in cells that will develop as nonsensory cell types. To determine the effects of retinoic acid signaling during the development of the organ of Corti, activation of retinoid receptors was blocked in cultures of the embryonic cochlea through receptor-specific antagonism or inhibition of retinoic acid synthesis. Results indicate that inhibition of retinoic acid signaling induces a significant decrease in the number of cells that develop as hair cells and a disruption in the development of the organ of Corti. These results demonstrate that cells within the developing cochlea can respond to retinoic acid and that signaling by retinoic acid is necessary for the normal development of the organ of Corti.

AB - The cellular mosaic of the mammalian organ of Corti represents one of the most highly ordered structures in any vertebrate system. A single row of inner hair cells and three or four rows of outer hair cells extend along the basal-to-apical axis of the cochlea. The factors that play a role in the development of specific cell types within the cochlea are largely unknown; however, the results of previous studies have strongly suggested that retinoic acid plays a role in the development of cells as hair cells. To determine whether cochlear progenitor cells can respond directly to retinoic acid, the expression patterns for each of the RAR and RXR receptors within the embryonic cochlear duct were determined by in situ hybridization. Results indicate that RARα, RXRα, and RXRγ are initially expressed throughout the cochlear duct. As development continues, the expression of each receptor becomes more intense in cells that will develop as hair cells. At the same time, receptor expression is down-regulated in cells that will develop as nonsensory cell types. To determine the effects of retinoic acid signaling during the development of the organ of Corti, activation of retinoid receptors was blocked in cultures of the embryonic cochlea through receptor-specific antagonism or inhibition of retinoic acid synthesis. Results indicate that inhibition of retinoic acid signaling induces a significant decrease in the number of cells that develop as hair cells and a disruption in the development of the organ of Corti. These results demonstrate that cells within the developing cochlea can respond to retinoic acid and that signaling by retinoic acid is necessary for the normal development of the organ of Corti.

KW - Cochlea

KW - Differentiation

KW - Hair cells

KW - Hearing

KW - Retinoids

UR - http://www.scopus.com/inward/record.url?scp=0033198473&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0033198473&partnerID=8YFLogxK

U2 - 10.1006/dbio.1999.9364

DO - 10.1006/dbio.1999.9364

M3 - Article

C2 - 10452855

AN - SCOPUS:0033198473

VL - 213

SP - 180

EP - 193

JO - Developmental Biology

JF - Developmental Biology

SN - 0012-1606

IS - 1

ER -