Retinoic Acid Pathway Regulation of Vascular Endothelial Growth Factor in Ovine Amnion

Cecilia Cheung, Debra Anderson, Marion Rouzaire, Loïc Blanchon, Vincent Sapin, Robert A. Brace

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Vascular endothelial growth factor (VEGF) has been proposed as an important regulator of amniotic fluid absorption across the amnion into the fetal vasculature on the surface of the placenta. However, the activators of VEGF expression and action in the amnion have not been identified. Using the pregnant sheep model, we aimed to investigate the presence of the retinoic acid (RA) pathway in ovine amnion and to determine its effect on VEGF expression. Further, we explored relationships between RA receptors and VEGF and tested the hypothesis that RA modulates intramembranous absorption (IMA) through induction of amnion VEGF in sheep fetuses subjected to altered IMA rates. Our study showed that RA receptor isoforms were expressed in sheep amnion, and RA response elements (RAREs) were identified in ovine RARβ and VEGF gene promoters. In ovine amnion cells, RA treatment upregulated RARβ messenger RNA (mRNA) and increased VEGF transcript levels. In sheep fetuses, increases in IMA rate was associated with elevated VEGF mRNA levels in the amnion but not in the chorion. Further, RARβ mRNA was positively correlated with VEGF mRNA levels in the amnion and not chorion. We conclude that an RA pathway is present in ovine fetal membranes and that RA is capable of inducing VEGF. The finding of a positive relationship between amnion VEGF and RARβ during altered IMA rate suggests that the retinoid pathway may play a role through VEGF in regulating intramembranous transport across the amnion.

Original languageEnglish (US)
JournalReproductive Sciences
DOIs
StateAccepted/In press - Jan 1 2018

Fingerprint

Amnion
Tretinoin
Vascular Endothelial Growth Factor A
Sheep
Chorion
Messenger RNA
Retinoic Acid Receptors
Fetus
Extraembryonic Membranes
Retinoids
Response Elements
Amniotic Fluid
Placenta
Protein Isoforms

Keywords

  • amniotic fluid
  • intramembranous transport
  • RAR and RXR
  • sheep fetal membranes

ASJC Scopus subject areas

  • Obstetrics and Gynecology

Cite this

Cheung, C., Anderson, D., Rouzaire, M., Blanchon, L., Sapin, V., & Brace, R. A. (Accepted/In press). Retinoic Acid Pathway Regulation of Vascular Endothelial Growth Factor in Ovine Amnion. Reproductive Sciences. https://doi.org/10.1177/1933719118765979

Retinoic Acid Pathway Regulation of Vascular Endothelial Growth Factor in Ovine Amnion. / Cheung, Cecilia; Anderson, Debra; Rouzaire, Marion; Blanchon, Loïc; Sapin, Vincent; Brace, Robert A.

In: Reproductive Sciences, 01.01.2018.

Research output: Contribution to journalArticle

Cheung, Cecilia ; Anderson, Debra ; Rouzaire, Marion ; Blanchon, Loïc ; Sapin, Vincent ; Brace, Robert A. / Retinoic Acid Pathway Regulation of Vascular Endothelial Growth Factor in Ovine Amnion. In: Reproductive Sciences. 2018.
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